Synthesis of 3'- and 3',5'-modified oligonucleotides on functionalized silica Silochrom-2
DOI:
https://doi.org/10.7124/bc.000760Keywords:
oligonucleotide conjugates, fluorescent labels, solidphase synthesis, functionalizationAbstract
The preparation of 3'-mono- and 3', 5' -bis-labeled oligonucleotides on a new fluorescein-functionalized polymer support based on Silochrom-2 microspherical silica is described. Oligonucleotide (dT)15 containing a dye residue at 3'-end was synthesized on this support by solid-phase phosphoramidite method. Post-synthetic 5'-modification of 3'-conjugate allowed obtaining double dye-labeled oligomer. In the future it will be used for the synthesis of fluorescent probe for nucleic acids detection.References
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