Isolation of individual proteins from 50S subparticles of Thermus thermophilus ribosomes. crystallization of TL7 protein
DOI:
https://doi.org/10.7124/bc.0001EBAbstract
A method of selective extraction is suggested for eight proteins of 50S subparticles of ribosomes from extremal-thermophilic bacterium Thermus thermophilus at 60 °C in the presence of 0.5 M and 1 M NH4C1 and 50 % ethanol. As a result of chromatographic separation of extracted proteins on CM-sepharose CL five individual proteins (the purity is not less than 95 %) have been obtained. One of them, TL7 (probably, an analog of L6 protein from E. coli ribosomes), has been crystallized by the method of «hanging» drop with ammonium sulfate as a precipitant.References
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