Organ-specific and light-inducible expression of genes in transgenic plants: receiving and cloning root-, tuber- and leaf-specific promoters

Authors

  • L. G. Ljoshina Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine 1, Murmans'ka Str., Kyiv, Ukraine, 02094 Author
  • T. V. Medvedeva Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine 1, Murmans'ka Str., Kyiv, Ukraine, 02094 Author
  • O. V. Bulko Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine 1, Murmans'ka Str., Kyiv, Ukraine, 02094 Author
  • A. P. Galkin Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine 1, Murmans'ka Str., Kyiv, Ukraine, 02094 Author
  • V. P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine 1, Murmans'ka Str., Kyiv, Ukraine, 02094 Author

DOI:

https://doi.org/10.7124/bc.00064A

Abstract

In this study the regulatory sequences ensuring organ-specific and light-dependent expression of several chimerical genes in bacterial and plant cells have been received and investigated. A fragment corresponding to the 5'-end sequence of the chloroplast ribosomal gene S12 (rpS12), a regulatory fragment of the patatin gene λpatl22, a promoter sequence of the gene of small subunit of ribulose-1,5-disphosphate carboxylase (rbcS3A) have been extracted and cloned in vectors containing marker genes. The organ-specific activity of the cloned promoters has been shown. The metallo-thioneine gene has been introduced into the vector constructed. The increased ability of transgenic plants to accumulate the ions of heavy metals in organ's cells with selective promoter-induced expression of the metallothioneine gene has been demonstrated.

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Published

2003-03-20

Issue

Vol. 19 No. 2 (2003)

Section

Molecular and Cell Biotechnologies