Replication of recombinant DNA in tobacco protoplasts

Authors

  • V. A. Kordyum Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author
  • V. A. Trukhanov Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author
  • E. K. Toporova Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author
  • V. N. Shulzhenko Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author
  • T. N. Checheneva Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author

DOI:

https://doi.org/10.7124/bc.0001E5

Abstract

Replication in tobacco protoplasts of vector including fragments of previously known vectors and elements of higher plants genome was detected.

References

Matzke MA, Susani M, Binns AN, Lewis ED, Rubenstein I, Matzke AJ. Transcription of a zein gene introduced into sunflower using a Ti plasmid vector. EMBO J. 1984;3(7):1525-31.

Horsch RB, Fraley RT, Rogers SG, Sanders PR, Lloyd A, Hoffmann N. Inheritance of functional foreign genes in plants. Science. 1984;223(4635):496-8.

Koncz C, Kreuzaler F, Kalman Z, Schell J. A simple method to transfer, integrate and study expression of foreign genes, such as chicken ovalbumin and alpha-actin in plant tumors. EMBO J. 1984;3(5):1029-37.

Wetzer WJ. Agrigenetics Researchers Express Plant Genes in Tobacco Plantlets. Nature Biotechnology. 1983; 1(6):461-462.

Barton KA, Brill WJ. Prospects in plant genetic engineering. Science. 1983;219(4585):671-82.

Gronenborn B. Cauliflower mosaic virus: a possible vector for genetic engineering in plants. Hoppe-Seyler's Z Physiol Chem. 1984; 365(3):217-218.

Hull R. Genetic engineering in plants. The possible use of cauliflower mosaic virus DNA as a vector. Plant cell cult, results and perspectives. Amsterdam: Elsevier, 1980:219-224.

Hull R, Davies JW. Genetic engineering with plant viruses, and their potential as vectors. Adv Virus Res. 1983;28:1-33.

Meshi T, Ishikawa M, Ohno T, Okada Y, Sano T, Ueda I, Shikata E. Double-stranded cDNAs of hop stunt viroid are infectious. J Biochem. 1984;95(5):1521-4.

Neuhaus G, Neuhaus G, Schweiger HG et al. Expression of zein genes in acetabularia mediterranea. Eur J Cell Biol. 1984; 33(5):26.

Gallun E, Raveh D. In vitro culture of tobacco protoplasts: survival of haploid and diploid protoplasts exposed to X-ray radiation at different times after isolation. Radiat Bot. 1975. 15(1):79-82.

Gleba YuYu, Shvydkaya LG, Butenko RG, Sytnik KM. Culture of isolated protoplasts. Fiziologiia rasteniy. 1974; 21(3):598-605.

Toporova EK, Checheneva TN, Kunakh VA i dr. Study penetration capability and state of expression of the plasmid DNA into tobacco protoplasts. Molekulyarnaya biologiya. 1982; Is. 32:39-44.

Checheneva TN, Voytiuk LI, Kunakh VA. Culturing Nicotiana tabacum protoplasts in the presence of antibiotics. Molekulyarnaya biologiya. 1979; Is. 24:58-62.

Clewell DB, Helinski DR. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969;62(4):1159-66.

Toporova EK, Checheneva TN, Trukhanov VA, Kostromina VV, Kordium VA. Fate of plasmid DNA introduced into tobacco protoplasts. Dokl Akad Nauk SSSR. 1983;269(5):1212-4.

biopolym.cell-1987-3-3-cover.png

Downloads

Published

1987-05-20

Issue

Vol. 3 No. 3 (1987)

Section

Short Communications