Investigation of DNA topoisomerase I as an autoantigen at the thyroid cancer

Authors

  • L. L. Sidorik Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • T. A. Rybkinska Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • O. G. Vigontina Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • M. I. Vudmaska Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • V. G. Naidenov Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • P. V. Pogribnyi R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine 45, Vasilkivska Str., Kyiv, Ukraine, 01022 Author
  • I. L. Lisovskii R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine 45, Vasilkivska Str., Kyiv, Ukraine, 01022 Author
  • L. P. Schvachko Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • I. Bronstein The University of York Heslington, York, YO10 5DD, UK Author
  • V. V. Lyzogubov Pathomorphological Laboratory "BIONTEK" 52A/88, Komsomol'ska Str., Dnipropetrovs'k, Ukraine, 49000 Author
  • E. A. Prihod'ko Pathomorphological Laboratory "BIONTEK" 52A/88, Komsomol'ska Str., Dnipropetrovs'k, Ukraine, 49000 Author
  • V. S. Usenko Pathomorphological Laboratory "BIONTEK" 52A/88, Komsomol'ska Str., Dnipropetrovs'k, Ukraine, 49000 Author
  • G. Kh. Matsuka Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author

DOI:

https://doi.org/10.7124/bc.0005DF

Abstract

The data concerning DNA-Topoisomerase I (Topo-I) from human placenta as an autoantigen at the thyroid cancer are presented. A new effective scheme of the Topo-I purification using the High-Trap columns is described, as well as the enzyme physico-chemical properties and its immunoreactivity in the ELJSA and immunoblotting. It was shown that 46 % of the sera from patients suffering the thyroid cancer were immunoreactive against Topo-I, 36 % of autoantibodies from thyroid cancer patients sera recognized sequential determinants on the Topo-I molecule. The highest levels of immunoreacivity were observed in the sera of thyroid cancer patients accompanied by autoimmune thyroiditis. We suggest a possible linkage between the autoimmune processes and malignization of the thyroid gland cells.

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Published

2001-11-20

Issue

Vol. 17 No. 6 (2001)

Section

Molecular Biomedicine