Expression of T-region genes in somatic cell hybrids obtained by fusion of normal and tumour cells
DOI:
https://doi.org/10.7124/bc.000018Abstract
Protoplast fusion of Nicotiana tabacum (56S3) tumour cells and Atropa belladonna L. mesophyll leaf cells was carried out. 57 hybrid cell lines in hormone-free medium are selected. Analysis of hybrid lines involved the determination of the multiple molecular forms of amylase and the LpDH activity, suppression of shoot and root organogenesis, resistance to 5-bromodeoxyuridine, 2-aminoethylcystein, 5-methyltryptophan as well as cytogenetic study. The available data permit demonstrating that under somatic cell hybridization most of tumour characters, controlled by T-DNA, are inherited as dominant. In some cases the expression of these characters depends on exogenous phytohormone presence or absence in nutrient media.References
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