Study of tobacco mosaic virus by atomic force microscope

Authors

  • V. P. Polischuk Taras Shevchenko National University of Kyiv 64, Volodymyrska Str., Kyiv, Ukraine, 01033 Author
  • O. V. Rengevych V. Ye. Lashkaryov Institute of Semiconductor Physics, NAS of Ukraine 41, Prospect Nauki, Kyiv, Ukraine, 03028 Author
  • G. V. Beketov V. Ye. Lashkaryov Institute of Semiconductor Physics, NAS of Ukraine 41, Prospect Nauki, Kyiv, Ukraine, 03028 Author
  • T. P. Tyvonchuk Institute of Agroecology of NAAS 12 Metrologichna Str., Kyiv, Ukraine, 03680 Author
  • A. L. Boyko Taras Shevchenko National University of Kyiv 64, Volodymyrska Str., Kyiv, Ukraine, 01033 Author

DOI:

https://doi.org/10.7124/bc.00053F

Abstract

The paper presents the methodological approaches to the use of an atomic force microscope to study the viral nature of objects and their interaction with specific immunoglobulin (IgG). Application formvar coating mica chips enhances the adsorption of virions virtually affecting the surface topography image of the phase shift STM interaction with IgG, which is proportional to Strength of adhesion of the probe to the surface, allowed visualization of the distribution of surface antibodies viral particles. The method is unique in terms of virology, as it removes the fixation preparation required for research in the transmission microscope

References

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missed

Binnig G, Quate CF, Gerber C. Atomic force microscope. Phys Rev Lett. 1986;56(9):930-933.

Kasas S, Ikai A. A method for anchoring round shaped cells for atomic force microscope imaging. Biophys J. 1995;68(5):1678-80.

Radmacher M, Cleveland JP, Fritz M, Hansma HG, Hansma PK. Mapping interaction forces with the atomic force microscope. Biophys J. 1994;66(6):2159-65.

Radmacher M., Fritz M., Cleveland J.P., Walters D.A., Hansma P.K. Imaging adhesion forces and elasticity of lysozyme adsorbed on mica with the atomic force microscope. Langmuir, 1994;10(10):3809-3814.

Nagao E., Dvorak J.A. Developing the atomic force microscope for studies of living cells. Int. Lab, 1999 (1):21-23.

Thomson NH, Fritz M, Radmacher M, Cleveland JP, Schmidt CF, Hansma PK. Protein tracking and detection of protein motion using atomic force microscopy. Biophys J. 1996;70(5):2421-31.

Hansma HG, Laney DE, Bezanilla M, Sinsheimer RL, Hansma PK. Applications for atomic force microscopy of DNA. Biophys J. 1995;68(5):1672-7.

Muller DJ, Schabert FA, B?ldt G, Engel A. Imaging purple membranes in aqueous solutions at sub-nanometer resolution by atomic force microscopy. Biophys J. 1995;68(5):1681-6.

Karrasch S, Hegerl R, Hoh JH, Baumeister W, Engel A. Atomic force microscopy produces faithful high-resolution images of protein surfaces in an aqueous environment. Proc Natl Acad Sci U S A. 1994;91(3):836-8.

Florin EL, Moy VT, Gaub HE. Adhesion forces between individual ligand-receptor pairs. Science. 1994;264(5157):415-7.

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Published

1999-09-20

Issue

Vol. 15 No. 5 (1999)

Section

Methods