Decreasing of glucose level in vitro and in vivo by recombinant vector containing human preproinsulin gene

Authors

  • F. Ajamian Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • E. M. Suhorada Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • T. A. Ruban Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • T. A. Titok Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author

DOI:

https://doi.org/10.7124/bc.000529

Abstract

The expression of human preproinsulin gene was studied. The fragment of genomic DNA, containing the sequences of preproinsulin gene without its personal promoter was cloned in the vector plasmid DNA pTR-UF under control of cytomegalovirus promoter. The obtained recombinant plasmid DNA was tested in vitro, in Hep-2 of tissue culture and in vivo at streptozotocin diabetic mice by introducing the preparation into the liver. As a result of our experiment we found out the reduction of glucose level both in the cells of tissue culture and in the blood of the experimental animals. The presented results, in the author's opinion, evidence about the availability of the expression of the human preproinsulin gene.

References

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Published

1999-07-20

Issue

Section

Structure and Function of Biopolymers