Biopolym. Cell. 2013; 29(2):143-149.
Viruses and Cell
Phylogenetic analysis of influenza A(H1N1)pdm viruses isolated in Ukraine during the 2009–2010 pandemic season
1, 2Leibenko L. V., 1Polischuk V. P., 2Mironenko A. P.
  1. Educational and Scientific Center "Institute of Biology",
    Taras Shevchenko National University of Kyiv
    64/13, Volodymyrska Str., Kyiv, Ukraine, 01601
  2. Gromashevsky L.V. Institute of Epidemiology and Infection Diseases, AMS of Ukraine
    5, Amosova Str., Kyiv, Ukraine, 03038

Abstract

Aim. To perform the phylogenetic analysis of segments, encoding hemagglutinin and neuraminidase of A(H1N1) pdm influenza viruses, isolated in Ukraine. Methods. In this study the real-time polymerase chain reaction (RT-PCR), sequencing and phylogenetic analysis methods were used. Results. Key mutations in amino acid sequences of proteins of Ukrainian pandemic influenza isolates were analyzed. High genetic similarity of Ukrainian and foreign pandemic isolates (99 %) was observed. Conclusions. The stability of Ukrainian isolates genes during 2009–2010 pandemic season was shown.
Keywords: phylogenetic analysis, nucleotide sequence, A(H1N1)pdm influenza viruses

References

[1] Gordon S. Avian influenza: a wake-up call from birds to humans Cleve Clin. J. Med 2004 71, N 4 P. 273–274.
[2] Kaiser J. Public health. Pandemic or not, experts welcome Bush flu plan Science 2005 310, N 5750 P. 952–953.
[3] Wan H., Sorrell E. M., Song H., Hossain M. J., Ramirez-Nieto G., Monne I., Stevens J., Cattolj G., Capua I., Chen L. M., Donis R. O., Busch J., Paulson J. C., Brockwell C., Webby R., Blanco J., Al-Natour M. Q., Perez D. R. Replication and transmission of H9N2 influenza viruses in ferrets: evaluation of pandemic potential PLoS ONE 2008 3, N 8 e2923.
[4] Strategic advisory group of experts on immunization – report of the extraordinary meeting on the influenza A (H1N1) 2009 pandemic (7 July 2009); Human infection with pandemic A (H1N1) 2009 influenza virus: clinical observations in hospitalized patients, Americas (July 2009 – update) Weekly Epidemiological Record (WHO) 2009 84, N 30 P. 301–308.
[5] Mironenko A. P., Onishchenko O. V., Golubka O. S., Kurinko N. A., Leibenko L. V. Pandemia of influenza in Ukraine in 2009–2010 winter season: lessons learned for future Liky Ukrainy 2010 N 10 (146) P. 86–90.
[6] Ma W., Lager K. M., Vincent A. L., Janke B. H., Gramer M. R., Richt J. A. The role of swine in the generation of novel influenza viruses Zoonoses Public Health 2009 56, N 6–7 P. 326–337.
[7] Smith G. J., Vijaykrishna D., Bahl J., Lycett S. J., Worobey M., Pybus O. G., Ma S. K., Cheung C. L., Raghwani J., Bhatt S., Peiris J. S., Guan Y., Rambaut A. Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic Nature 2009 459, N 7250 P. 1122–1125.
[8] Nava G. M., Attene-Ramos M. S., Ang J. K., Escorcia M. Origins of the new influenza A(H1N1) virus: time to take action Eurosurveillance 2009 14, N 22 pii: 19228.
[9] McHardy A. C., Adams B. The role of genomics in tracking the evolution of influenza A virus PLoS Pathog 2009 5, N 10 e1000566.
[10] Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods Mol. Biol. Evol 2011 28, N 10 P. 2731– 2739.
[11] Saitou N., Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees Mol. Biol. Evol 1987 4, N 4 P. 406–425.
[12] Kimura M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences J. Mol. Evol 1980 16, N 2 P. 111–120.
[13] Felsenstein J. Confidence limits on phylogenies: An approach using the bootstrap Evolution 1985 39, N 4 P. 783–791.
[14] Galiano M., Agapow P. M., Thompson C., Platt S. Underwood A., Ellis J., Myers R., Green J., Zambon M. Evolutionary pathways of the pandemic influenza A (H1N1) 2009 in the UK PLoS One 2011 6, N 8 e23779.
[15] Danishuddin M., Khan S. N., Khan A. U. Phylogenetic analysis of surface proteins of novel H1N1 virus isolated from 2009 pandemic Bioinformation 2009 4, N 2 P. 94–97.
[16] Han L., Lu W., Han Y., Li S., Yin J., Xie J., Su T., Cao G. Evolutionary characteristics of swine-origin H1N1 influenza virus that infected humans from sporadic to pandemic J. Public Health and Epidemiol 2011 3, N 6 P. 254–270.
[17] Mak G. C., Au K. W, Tai L. S., Chuang K. C., Cheng K. C., Shiu T. C., Lim W. Association of D222G substitution in haemagglutinin of 2009 pandemic influenza A (H1N1) with severe disease Euro. Surveill 2010 15, N 14 pii 19534.
[18] Puzelli S., Facchini M., Spagnolo D., De Marco M. A., Calzoletti L., Zanetti A., Fumagalli R., Tanzi M. L., Cassone A., Rezza G., Donatelli I., Surveillance Group for Pandemic A H1N1 2009 Influenza Virus in Italy. Transmission of hemagglutinin D222G mutant strain of pandemic (H1N1) 2009 virus Emerg. Infect. Dis 2010 16, N 5 P. 863–865.
[19] Liu Y., Wang J., Ji J., Chang S., Xue C., Ma J., Bi Y., Xie Q. Phylogenetic diversity and genotypic complexity of H1N1 subtype swine influenza viruses isolated in Mainland China Virol. J 2012 9 P. 289.
[20] Herlocher M. L., Truscon R., Elias S., Yen H. L., Roberts N. A., Ohmit S. E., Monto A. S. Influenza viruses resistant to the antiviral drug oseltamivir: transmission studies in ferrets J. Infect. Dis 2004 190, N 9:1627–1630.
[21] Lackenby A., Hungnes O., Dudman S. G., Meijer A., Paget W. J., Hay A. J., Zambon M. C. Emergence of resistance to oseltamivir among influenza A(H1N1) viruses in Europe Euro. Surveill 2008 13, N 5 pii: 8026.
[22] Leibenko L., Babii S., Kurinko N., Mironenko A. A four-year survey of the oseltamivir-resistant viruses in Ukraine 3rd Int. Influenza Meet. (2–4 Sept. 2012, Munster, Germany) Munster, 2012 P. 108.
[23] Updated interim recommendations for obstetric health care providers related to use of antiviral medications in the treatment and prevention of influenza for the 2009–2010 season (September 17, 2009) Atlanta, 2009.
[24] Leung T. W., Tai A. L., Cheng P. K., Kong M. S., Lim W. Detection of an oseltamivir-resistant pandemic influenza A/H1N1 virus in Hong Kong J. Clin.Virol 2009 46, N 3 P. 298–299.