Biopolym. Cell. 2003; 19(1):31-36.
Structure and Function of Biopolymers
Kinetic properties of the Penicilium vitale catalase. Catalytic reaction of the enzyme
- Palladin Institute of Biochemistry, NAS of Ukraine
9, Leontovycha Str., Kyiv, Ukraine, 01601
Abstract
The fast kinetics of the P. vitale catalase (CPV) catalytic reaction was investigated by the stop-flow technique. The transient-state rate constants of the intermediate complex (Compound I) formation as well as the rate of spontaneous dissociation and dissociation resulting in the reaction product creation were found at 25 °C to be 0.614·106 M-1s-1, 1.78 s-1 and 1.85·106 M-1s-1, respectively.
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