Biopolym. Cell. 2000; 16(6):525-529.
http://dx.doi.org/10.7124/bc.000593
Cell Biology
The expression of human defensine-6 (HD-6) gene in transformed human cell lines
1Lisovsky I. L., 1Khobta O. I., 1Soldatkina M. A., 1Litvin D. I., 1Markeeva N. V., 2Sidorik L. L., 1Pogrebnoy P. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
    45, Vasilkivska Str., Kyiv, Ukraine, 01022

Abstract

The expression of human defensine-6 (HD-6) gene was studied in transformed human cell lines of different histogenesis: A431 (epidermoid carcinoma), CAM (T-cell lymphoma), Molt-4 (T-cell lymphoma), KB (epidermoid carcinoma), HeLa (epithelioid carcinoma), Colo 320HSR (adenocarcinoma), U251 (glioblastoma). HD-6 gene expression was recorded by RT-PCR in all cell lines
Full text: (PDF, in Russian)

References

[1] Boman HG. Peptide antibiotics and their role in innate immunity. Annu Rev Immunol. 1995;13:61-92. Review.
[2] Diamond G, Jones DE, Bevins CL. Airway epithelial cells are the site of expression of a mammalian antimicrobial peptide gene. Proc Natl Acad Sci U S A. 1993;90(10):4596-600.
[3] Ganz T, Lehrer RI. Antimicrobial peptides of vertebrates. Curr Opin Immunol. 1998;10(1):41-4.
[4] Ganz T, Lehrer RI. Defensins. Curr Opin Immunol. 1994;6(4):584-9.
[5] Stolzenberg ED, Anderson GM, Ackermann MR, Whitlock RH, Zasloff M. Epithelial antibiotic induced in states of disease. Proc Natl Acad Sci U S A. 1997;94(16):8686-90.
[6] Diamond G, Russell JP, Bevins CL. Inducible expression of an antibiotic peptide gene in lipopolysaccharide-challenged tracheal epithelial cells. Proc Natl Acad Sci U S A. 1996;93(10):5156-60.
[7] Harder J, Bartels J, Christophers E, Schr?der JM. A peptide antibiotic from human skin. Nature. 1997;387(6636):861.
[8] Bensch KW, Raida M, M?gert HJ, Schulz-Knappe P, Forssmann WG. hBD-1: a novel beta-defensin from human plasma. FEBS Lett. 1995;368(2):331-5.
[9] Valore EV, Park CH, Quayle AJ, Wiles KR, McCray PB Jr, Ganz T. Human beta-defensin-1: an antimicrobial peptide of urogenital tissues. J Clin Invest. 1998;101(8):1633-42.
[10] McNAMARA NA, Van R, Tuchin OS, Fleiszig SM. Ocular surface epithelia express mRNA for human beta defensin-2. Exp Eye Res. 1999;69(5):483-90.
[11] Wada A, Mori N, Oishi K, Hojo H, Nakahara Y, Hamanaka Y, Nagashima M, Sekine I, Ogushi K, Niidome T, Nagatake T, Moss J, Hirayama T. Induction of human beta-defensin-2 mRNA expression by Helicobacter pylori in human gastric cell line MKN45 cells on cag pathogenicity island. Biochem Biophys Res Commun. 1999;263(3):770-4.
[12] Abiko Y, Mitamura J, Nishimura M, Muramatsu T, Inoue T, Shimono M, Kaku T. Pattern of expression of beta-defensins in oral squamous cell carcinoma. Cancer Lett. 1999;143(1):37-43.
[13] Kagan BL, Selsted ME, Ganz T, Lehrer RI. Antimicrobial defensin peptides form voltage-dependent ion-permeable channels in planar lipid bilayer membranes. Proc Natl Acad Sci U S A. 1990;87(1):210-4.
[14] White SH, Wimley WC, Selsted ME. Structure, function, and membrane integration of defensins. Curr Opin Struct Biol. 1995;5(4):521-7.
[15] Hristova K, Selsted ME, White SH. Interactions of monomeric rabbit neutrophil defensins with bilayers: comparison with dimeric human defensin HNP-2. Biochemistry. 1996;35(36):11888-94.
[16] Chertov O, Michiel DF, Xu L, Wang JM, Tani K, Murphy WJ, Longo DL, Taub DD, Oppenheim JJ. Identification of defensin-1, defensin-2, and CAP37/azurocidin as T-cell chemoattractant proteins released from interleukin-8-stimulated neutrophils. J Biol Chem. 1996;271(6):2935-40.
[17] Lillard JW Jr, Boyaka PN, Chertov O, Oppenheim JJ, McGhee JR. Mechanisms for induction of acquired host immunity by neutrophil peptide defensins. Proc Natl Acad Sci U S A. 1999;96(2):651-6.
[18] Proh?szka Z, N?met K, Csermely P, Hudecz F, Mez? G, F?st G. Defensins purified from human granulocytes bind C1q and activate the classical complement pathway like the transmembrane glycoprotein gp41 of HIV-1. Mol Immunol. 1997;34(11):809-16.
[19] Liu L, Zhao C, Heng HH, Ganz T. The human beta-defensin-1 and alpha-defensins are encoded by adjacent genes: two peptide families with differing disulfide topology share a common ancestry. Genomics. 1997;43(3):316-20.
[20] Maeda H, Akaike T. Nitric oxide and oxygen radicals in infection, inflammation, and cancer. Biochemistry (Mosc). 1998;63(7):854-65.
[21] McKaig RG, Baric RS, Olshan AF. Human papillomavirus and head and neck cancer: epidemiology and molecular biology. Head Neck. 1998;20(3):250-65.
[22] Poljak M, Cerar A, Seme K. Human papillomavirus infection in esophageal carcinomas: a study of 121 lesions using multiple broad-spectrum polymerase chain reactions and literature review. Hum Pathol. 1998;29(3):266-71.
[23] Sasaki H, Ishizuka T, Muto M, Nezu M, Nakanishi Y, Inagaki Y, Watanabe H, Watanabe H, Terada M. Presence of Streptococcus anginosus DNA in esophageal cancer, dysplasia of esophagus, and gastric cancer. Cancer Res. 1998;58(14):2991-5.
[24] Tahara E. Molecular mechanism of human stomach carcinogenesis implicated in Helicobacter pylori infection. Exp Toxicol Pathol. 1998;50(4-6):375-8.
[25] PogrebnoÄ­ PV, Serov SM, Klenchin VA, Luk'ianov AV, Garmanchuk LV, TarnavskiÄ­ DV, Markeeva NV, Bykorez AI. Expression of the gene for transforming growth factor type alpha, transferred into A431 cells by a recombinant retroviral vector. Mol Biol (Mosk). 1993;27(4):833-8.
[26] Pogrebnoy PV, Hobta AI, Soldatkina MA, Garmanchouk LV, Markeeva NV, Eremenko T. A protein kinase inhibitor from A431 subline overexpressing TGF alpha possesses antimicrobial activity. New Microbiol. 1998;21(3):269-73.
[27] Hanahan D, Weinberg RA. The hallmarks of cancer. Cell. 2000;100(1):57-70.
[28] Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162(1):156-9.
[29] Weinberg A, Krisanaprakornkit S, Dale BA. Epithelial antimicrobial peptides: review and significance for oral applications. Crit Rev Oral Biol Med. 1998;9(4):399-414.
[30] Zhao C, Wang I, Lehrer RI. Widespread expression of beta-defensin hBD-1 in human secretory glands and epithelial cells. FEBS Lett. 1996;396(2-3):319-22.
[31] Krisanaprakornkit S, Weinberg A, Perez CN, Dale BA. Expression of the peptide antibiotic human beta-defensin 1 in cultured gingival epithelial cells and gingival tissue. Infect Immun. 1998;66(9):4222-8.
[32] Wilson CL, Ouellette AJ, Satchell DP, Ayabe T, L?pez-Boado YS, Stratman JL, Hultgren SJ, Matrisian LM, Parks WC. Regulation of intestinal alpha-defensin activation by the metalloproteinase matrilysin in innate host defense. Science. 1999;286(5437):113-7.