Biopolym. Cell. 1998; 14(2):144-151.
http://dx.doi.org/10.7124/bc.0004C7
Structure and Function of Biopolymers
Comparative study of the physical and chemical properties of the tyrosyl-tRNA synthetases from Thermus thermophilus and Escherichia coli
1Egorova S. P., 1Yaremchuk A. D., 1Tukalo M. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

A comparative study of the physical and chemical properties of the tyrosyl-tRNA synthetases from T. thermophilus and E. coli has been carried out. KM values for tRNATyr from T. termophilus and E. coli, for L-tyrosine and ATP, as well as their temperature dependences were determined. The KCl effects were studied for the aminoacylation of the homologous tRNATyr and heterologous tRNATyr species. A comparative analysis of amino acid composition of the tyrosyl-tRNA synthetases from T. thermophilus and E. coli has been carried out. Temperature dependences of tRNA aminoacylation and [32P]PPi-ATP exchange rates and the thermostability curves for tyrosyl-tRNA synthetases from T. thermophilus and E. coli were determined.
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