Biopolym. Cell. 2012; 28(6):424-428.
http://dx.doi.org/10.7124/bc.000131
Structure and Function of Biopolymers
Generation of monoclonal antibodies specific to ribosomal protein S6 kinase 1
1Savinska L. O., 1Klipa O. M., 1Demchuk N. O., 1Ovcharenko G. V., 1Malanchuk O. M., 1Tykhonkova I. O., 1Palchevskyy S. S., 1Filonenko V. V.
  1. State Key Laboratory of Molecular and Cellular Biology
    Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The aim of this study was to produce monoclonal antibodies directed to the N-terminal regulatory region of S6K1, which shows very low homology to S6K2. Methods. Hybridoma technology, ELISA, Western blot, Immunoprecipitation. Results. Three hybridoma clones (A1, A2 and A3) producing mAbs specific to ribosomal protein S6 kinase 1 have been generated. Specificity of mAbs has been confirmed by Western blot and immunoprecipitation technique. Conclusions. The obtained antibodies are suitable for elucidating signal transduction pathways involving S6K1.
Keywords: ribosomal protein S6 kinase, mAbs
Full text: (PDF, in English)

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