Biopolym. Cell. 1989; 5(5):38-43.
http://dx.doi.org/10.7124/bc.0000E2
Structure and Function of Biopolymers
The effect of two types of binding of the ligands on the parameters of the DNA helix-coil transition
1Karapetyan A. T., 1, 2Vardevanyan P. O., 2Terzikyan G. A., 3Frank-Kamenetskii M. D.
  1. Kirovokan State Pedagogical Institute
    Kirovokan, USSR
  2. Yerevan State University, Department of Molecular Physics and Biophysics,
    Yerevan, USSR
  3. Institute of Molecular Genetics, Academy of Sciences of the USSR
    Moscow, USSR

Abstract

A theory of the helix-coil transition in DNA complexes with ligands is developed for the case of two types of interaction differing from each other in the binding constants of the ligand to the unmelted DNA. The formulas connecting the experimentally found parameters of the melting curves of the DNA-ligand complexes with the parameters of the theory are derived. It is shown that the formula for the enthalpy of the helix-coil transition of DNA (ΔH) is universal and valid at the very general assumptions.
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References

[1] Poletayev LI. Circular dichroism of DNA comPlexes. Itogi nauki i tekhniki. Moscow, VINITI, 1976; (Ser. molekulyarnaya biologiya; Vol 8; Pt 2). 180-241
[2] Wakelin LPG. Polyfunctional DNA intercalating agents. Med Res Rev. 1986;6(3):275-340.
[3] Zimmer C, Wahnert U. Nonintercalating DNA-binding ligands: specificity of the interaction and their use as tools in biophysical, biochemical and biological investigations of the genetic material. Prog Biophys Mol Biol. 1986;47(1):31-112.
[4] Ivanov VI, Minchenkova LE. The effect of transition metals on DNA thermal denaturation. Biokhimiia. 1965;30(6):1213-7.
[5] Blagoi YuP, Sorokin VA, Valeev VA, Gladchenko GO. Effects of calcium and manganese ions on the helix-coil transition of DNA. Biopolymers. 1983;22(7):1641-56.
[6] Frank-Kamenetskii MD. Theory of the helix - coil transition for deoxyribonucleic acids with additional connections between the chains. Vysokomolekulyarnye Soedineniya. 1965; 7(2):354-61.
[7] Frank-Kamenetskii MD. On the theory of melting DNA comPlexes with low molecular weight substances. Mol Biol (Mosk). 1972; 6(4): 621-627
[8] Vedenov AM, Dykhne AM, Frank-Kamenetskii MD. The helix-coil transition in DNA. Usp Fiz nauk. 1971; 105(3):479-519.
[9] Lando DIu, Krot VI, Frank-Kamenetskii MD. Fusion of complexes of DNA and extended ligands. Mol Biol (Mosk). 1975;9(6):856-60.
[10] Kapanetyan AT, Permagorov VI, Frank-Kamenetskii MD, Lazurkin YuS. Thermodynamic study of DNA comPlexes with dyes. Mol Biol (Mosk). 1972; 6(6): 867-73.
[11] Borisova OF, Grechko VV, Aleshkina LA, Kuznetsova NV. Study of the structural organization of RNA from phage MS2 using fluorescent dyes. Mol Biol (Mosk). 1984;18(6):1617-24.
[12] Borisova OF, Grechko VV, Kuznetsova NV, Sakharova NK, Timokhina GI. Comparison of the conformation of RNA from phage MS2 and 16S rRNA. Interaction with dyes specific for the secondary structure of native RNA and RNA subjected to hydrolysis by nuclease S1. Mol Biol (Mosk). 1987;21(2):515-28.
[13] Landau LD, Lifshits EM. Statistical Physics. Moscow, Nauka, 1964; 568 P.