Biopolym. Cell. 1993; 9(5):73-77.
Cell Biology
Genetic transformation of some Solanum and Lycopersicon wild species by leaf-discs method
1Rudas V. A., 1Zavgorodnya A. V., 1Cherep N. N., 1Gleba Yu. Yu.
  1. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

Using Agrobacterium tumefaciens-mediated transformation kanamycin-resistant plants of Lycopersicon peruvianutn var. humifusum, L. peruvianum Mill., L. pennellii, Solatium aculeatissimum, S. sisymbrifolium, S. gilo, S. mammosum were obtained. By this procedure plants of L. peruvianum var. dentatum, S. rickil, S. lycopersicoides, S. nigrum resistant to kanamycin, hygromycin as well as resistance to both kanamycin and hygromycin were obtained too. Assay of neornycin phosphotransferase (NPTII)-activity confirmed this chimaeric gene expression in the transformed plants.

References

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