Biopolym. Cell. 1992; 8(3):29-32.
http://dx.doi.org/10.7124/bc.000321
Structure and Function of Biopolymers
Analysis of rescued plasmid from the transgenic silkworm
1Nikolaev A. I., 2Tchkonia T. T., 2Eristavi-Kafiani K. A., 1Tarantul V. Z.
  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
    32, Vavilova Str., Moscow, Russian Federation, 119991
  2. Ivane Javakhishvili Tbilisi State University
    Chavchavdze Ave., 0179 Tbilisi, Georgia

Abstract

With the aid of microinjection of plasmid pPrC-LTRl,5. harboring 1.5 DNA copies of Rous sarcoma virus (RSV) long terminal repeats (LTRs) in vector pBR322, transgenic silkworms (Bombyx mori L.) were obtained. The transgene was transmitted in three, obtained up to now, generation. Most of the exogenous DNA is not integrated into the genome and persist as an extrachromosomal element which was subject to rearrangements. Plasmids have been rescued that carry the part of injected DNA and also fragments of the silkworm cellular DNA. In one of the rescued plasmids a sequence was found that is a member of a family of evolutionally concerved repeated sequences.
Full text: (PDF, in Ukrainian) (PDF, in Russian)

References

[1] Palmiter RD, Brinster RL. Germ-line transformation of mice. Annu Rev Genet. 1986;20:465-99. Review.
[2] Gordon K, Ruddle FH. Gene transfer into mouse embryos. Dev Biol (N Y 1985). 1986;4:1-36.
[3] Solomko AP, Ryndich AV, Titok TG, Morozova LM, Vagina IN, Chashchina LI, Evsykov SV, Kirichenko IV, Sarapina NA. The transgenic mice containing pATV-8 AND pBR322 plasmids. Biopolym Cell. 1988; 4(5):267-9.
[4] Rassoulzadegan M, Léopold P, Vailly J, Cuzin F. Germ line transmission of autonomous genetic elements in transgenic mouse strains. Cell. 1986;46(4):513–9.
[5] Nikolaev AI, Tchkonia TT, Kafiani KA. Introduction of heterologous DNA into early embryos of the silkworm. Ontogenez. 1989; 20(2):364-71.
[6] Nikolaev AI, Tchkonia TT, Kafiani-Eristavi KA. Extrachromosomal lokalization and transmittance of the recombinant plasmid, migroinjected into the silkworm grain. Mol Biol (Mosk). 1991; 25(5): 1136-45.
[7] A. s. 1336570 USSR. DNA microinjection method in insect eggs. AI Nikolaev, KA Kafiani. 1991.
[8] Gazaryan KG, Nabirochkin SD, Shibanova EN, Tatosyan AG, Kisselev FL, Ambartsumian NS, Tikhonenko TI, Goltzov VA. Unstable visible mutations induced in Drosophila melanogaster by injections of oncogenic virus DNA into the polar plasm of early embryos. Mol Gen Genet. 1987;207(1):130-41.
[9] Plasmids: A practical approach. Edited by K.G. Hardy. Oxford-Washington, DC: IRL Press, 1987; 180 pp.
[10] Tarantul VZ, Kucheriavy VV, Makarova IV, Baranov YuN, Begetova TV, Andreeva LE, Gazaryan KG. Rearrangements of microinjected recombinant DNA in the genome of transgenic mice. Mol Gen Genet. 1986;203(2):305-11.
[11] Makarova IV, Tarantul VZ, Gazarian KG. Structural features of the integration site of foreign DNA in the transgenic mouse genome. Mol Biol (Mosk). 1988;22(6):1553-61.
[12] Degroote F, Pont G, Micard D, Picard G. Extrachromosomal circular DNAs in Drosophila melanogaster: comparison between embryos and Kc0% cells. Chromosoma. 1989;98(3):201-6.
[13] Rush MG, Misra R. Extrachromosomal DNA in eucaryotes. Plasmid. 1985;14(3):177-91.
[14] Covarrubias L, Nishida Y, Mintz B. Early postimplantation embryo lethality due to DNA rearrangements in a transgenic mouse strain. Proc Natl Acad Sci U S A. 1986;83(16):6020-4.
[15] Tarantul VZ, Kuznetsova ED, Gazarian KG. Characteristic of segments of transgenic animal genomes, adjacent to integrated sequences of foreign DNA. Mol Biol (Mosk). 1989;23(4):1036-40.