Biopolym. Cell. 1992; 8(3):19-23.
Structure and Function of Biopolymers
Revelation and characterization of mitochondrial plasmid-like DNA of cotton
1Yusupov T. Yu., 1Nauruzbaeva R. M., 1, 2Khazratov P. R., 1Ibragimov A. P.
  1. Institute of Experimental Biology of Plants, Academy of Sciences of the Republic of Uzbekistan
    Uzbekistan
  2. NGO "Biologist",
    Tashkent, Uzbekistan

Abstract

Mitochondrial DNA was extracted from ethylated seedlings of cotton using the methods of differential and equilibrial ultra centrifugation in CsCl-Hoechst 33258 density gradient. By electron microscopy and agarose gel electrophoresis, it was shown that the mitochondrii of cotton belonging to G. hirsutum L. species (variety 108-F) has an addition to basic DNA of high molecular weight, two minicircular DNA of the size of 6.5 and 2.4 Kb. Analysis of the results of analytical ultra centrifugation and electrophoregrams of the distribution of the products of segregation of DNA of the cell organelles using restrictase EcoRI showed that the plasmid-like DNA has mitochondrial origin and that it does not contain significant impurities of nuclear and plastid DNA.

References

[1] Esser K, Kück U, Stahl U, Tudzynski P. Cloning vectors of mitochondrial origin for eukaryotes: A new concept in genetic engineering. Curr Genet. 1983;7(4):239-43.
[2] Shay J. Techniques in Somatic Cell Genetics. New York : Plenum Press, 1982. 541 p.
[3] Gleba YuYu, Sytnik KM. Protoplast fusion and genetic engineering of higher plants. Kyiv, Naukova Dumka, 1982; 104 p.
[4] Pring DR, Levings CS, Hu WW, Timothy DH. Unique DNA associated with mitochondria in the "S"-type cytoplasm of male-sterile maize. Proc Natl Acad Sci U S A. 1977;74(7):2904-8.
[5] Powling A. Species of small DNA molecules found in mitochondria from sugarbeet with normal and male sterile cytoplasms. Mol Gen Genet. 1981;183(1):82–4.
[6] Pring DR, Conde MF, Schertz KF, Levings CS. Plasmid-like DNAs associated with mitochondria of cytoplasmic male-sterile Sorghum. Mol Gen Genet. 1982;186(2):180–4.
[7] Nikiforova ID, Negruk VI. Comparative electrophoretical analysis of plasmid-like mitochondrial DNAs in Vicia faba and in some other legumes. Planta. 1983;157(1):81-4.
[8] Yusupov TYu, Irismetov AA, Rakhmatov NA, Ibragimov AP. Obtaining of pure DNA preparations from cellular cotton organoids and certain data on their structural organization. Fiziologiia i biokhimiia kul'turnykh rasteniy. 1985; 17(2):157-62.
[9] Schildkraut CL, Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962;4:430-43.
[10] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.
[11] Kleinschmidt AK. Monolayer techniques in electron microscopy of nucleic acid molecules. Methods Enzymol. 1968;12 B: 361-377.
[12] Yusupov TYu. Comparative study of structural organization of the chloroplast DNA of some species of cotton: Dis .... kand. biol. nauk. Tashkent, 1982. 119 p.