Biopolym. Cell. 1991; 7(5):103-107.
Genome and Its Regulation
Determination of replicative activity of recombinant plasmids carrying SV40 eukaryotic regulatory region in mammalian cell cultures
1Landau S. M., 1Tichonov A. V., 1Varzanova I. S., 1Zharova L. G.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

The recombinant plasmids replication in mammalian cell cultures has been employed as a method for estimating the functional activity of SV40 eukaryotic regulatory region. It was shown that recombinant plasmids examined replicate in mammalian cell cultures and therefore possess functional active SV40 regulatory region. This method can be used for the purposes of gene therapy.

References

[1] Lusky M, Botchan M. Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences. Nature. 1981;293(5827):79-81.
[2] Landau SM, Sasina LK, Chashchin NA, Chashchina LI. Studies in replication of plasmids containing SV40 genome in mammalian cells. Biopolym Cell. 1987; 3(3):134-6.
[3] Landau SM, Sasina LK, Shlyankevich MA, Dreeze OB. Studies in replication of plasmids containing a complete SV40 genome in permissive mammalian cells. Biopolym Cell. 1989; 5(2):94-7.
[4] O'Hare K, Benoist C, Breathnach R. Transformation of mouse fibroblasts to methotrexate resistance by a recombinant plasmid expressing a prokaryotic dihydrofolate reductase. Proc Natl Acad Sci U S A. 1981;78(3):1527-31.
[5] An G, Hidaka K, Siminovitch L. Expression of bacterial beta-galactosidase in animal cells. Mol Cell Biol. 1982;2(12):1628-32.
[6] Sasina LK, Landau SM, Kordium VA. Construction and analysis of two-replicon hybrid plasmids containing the genome of simian papovavirus SV40. Mol Gen Mikrobiol Virusol. 1986;(1):26-30.
[7] Landau SM, Nosach LN, Pavlova GV. Morphological method for estimation of simian virus 40 infectious titer. Arch Virol. 1982;73(1):79-84.