Biopolym. Cell. 1990; 6(3):31-39.
Structure and Function of Biopolymers
Low-frequency phonon raman spectra of nucleic acid constituents: pyrimidine bases
1Govorun D. N., 1Mishchuk Ya. R., 1Zheltovsky N. V.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

Low-frequency (10–15 cm-1 < v < 200 cm-1) Raman spectra of polycrystalline cytosine, uracil and thymine at 300 K has been investigated in detail. Lattice vibration bands are identified, and types of symmetry are determined in some cases. Possible applications of obtained results in the field of biophysics are discussed. The authors point out the advantage of using Raman spectroscopy on external degrees of freedom to study structural-dynamical properties of the solid biomolecular complexes as well as for analytical purposes.

References

[1] Govorun DN, Mishchuk YaR, Zheltovskiy NV. Low-frequency phonon Raman spectra of nucleic acid constituents: purine bases. Biopolym Cell. 1990; 6(3):24-31.
[2] Barker DL, Marsh RE. The crystal structure of cytosine. Acta Cryst 1964;17(12):1581–7.
[3] Susi H, Ard JS, Purcell JM. Vibrational spectra of nucleic acid constituents—II. Spectrochim Acta A. 1973;29(4):725–33.
[4] Susi H, Ard JS. Planar valence force constants and assignments for pyrimidine derivatives. Spectrochim Acta A. 1974;30(9):1843–53.
[5] Beetz CP, Ascarelli G. The low frequency vibrations of pyrimidine and purine bases. Spectrochim Acta A. 1980;36(3):299–313.
[6] Maleev VYa, Ctanevich AYe. The absorption spectra of nitrogenous bases crystals in the far infrared. Spectroscopy in solids. Leningrad, Nauka, 1969; Vol. 4. 280 p.
[7] Lord RC, Thomas GJ. Raman spectral studies of nucleic acids and related molecules—I Ribonucleic acid derivatives. Spectrochim Acta A. 1967;23(9):2551–91.
[8] Mathlouthi M, Seuvre AM, Koenig JL. F.t.-i.r. and laser-Raman spectra of cytosine and cytidine. Carbohydr Res. 1986;146(1):1-13.
[9] Babkov LM, Puchkovskaya GA, Makarenko SP, Gavrilko TA. IR spectroscopy of molecular hydrogen-bonded crystals. Kyiv, Naukova Dumka, 1989; 160 p.
[10] Kugel GE, Gerbaux X, Carabatos C, Martel P, Powell BM. Low-frequency vibrational Raman and i.r. spectrum of crystalline cystosine monohydrate. Spectrochim Acta A. 1979;35(10):1155–63.
[11] Jeffrey GA, Kinoshita Y. The crystal structure of cytosine monohydrate. Acta Cryst. 1963;16(1):20–8.
[12] Stewart RF, Jensen LH. Redetermination of the crystal structure of uracil. Acta Cryst. 1967;23(6):1102–5.
[13] Gerdil R. The crystal structure of thymine monohydrate. Acta Cryst. 1961;14(4):333–44.
[14] Susi H, Ard JS. Vibrational spectra of nucleic acid constituents—I. Planar vibrations of uracil. Spectrochim Acta A. 1971;27(9):1549–62.
[15] Bandekar J, Zundel G. Normal coordinate analysis treatment on uracil in solid state. Spectrochim Acta A. 1983;39(4):343–55.
[16] Wójcik MJ. Low-frequency vibrational spectra of crystalline uracil, thymine and their 1-methyl-derivatives. J Mol Struct. 1988;189(1-2):239–42.
[17] Shimanouchi T, Harada I. Far-Infrared Spectra of Cyanuric Acid, Uracil, and Diketopiperazine. J Chem Phys. 1964;41(9):2651-5.
[18] Lewis TP, Miles HT, Becker ED. Infrared and Raman spectra and vibrational assignments for 1-methyluracil and isotopic derivatives. J Phys Chem. 1984;88(15):3253–60.
[19] Colombo L., Kirin D. Prednost upotrebe monokristalinicnich uzoraka u ramanskoj spekiroskopiji. Primjer 1-metil-uracila. Vestn. Sloven. Kem. drus. 1986; (33):31-36.
[20] Colombo L, Kirin D. Raman spectrum of 1-methyl-uracil single crystal. Interpretation of internal and external spectra. Spectrochim Acta A. 1986;42(4):557–65.
[21] Kirin D, Colombo L, Furić K, Meier W. Low-frequency vibrational spectrum of the 1-methyl-thymine single crystal. Spectrochim Acta A. 1975;31(11):1721–7.
[22] Hoogsteen K. The crystal structure of 1-methylthymine. Acta Crys. 1963;16(1):28–38.
[23] Harada I, Lord RC. Low-frequency infrared and Raman spectra of some adenine and uracil crystals. Spectrochim Acta A. 1970;26(12):2305–18.
[24] Kyogoku Y, Higuchi S, Tsuboi M. Intra-red absorption spectra of the single crystals of 1-methyl-thymine, 9-methyladenine and their 1:1 complex. Spectrochim Acta A. 1967;23(4):969–83.