Biopolym. Cell. 1988; 4(2):75-78.
Структура и функции биополимеров
Избирательное введение спин-метки в остаток N-ацетилнейраминовой кислоты олигосахаридной группировки Fab-области иммуноглобулина М
1Тимофеев В. П., 2Лапук В. А., 2Чухрова А. И., 1Арутюнян А. Е., 2Киселева Т. М.
  1. Институт молекулярной биологии АН СССР
    Москва, СССР
  2. Институт органической химии им. Н. Д. Зелинского АН СССР
    Москва, СССР

Abstract

Предложена методика введения спин-метки (2,2,6,6-тетраметил-4-аминопиперидин-1-оксил) в остаток N-ацетилнейраминовой кислоты олигосахаридной группировки Fab-области IgM.

References

[1] Timofeev VP. Segmental mobility of poly (s) and the spin-label method. Mol biol (Mosk). 1986; 20(3):697-711.
[2] Timofeev VP, Lapuk VA. Study of the irreversible conformation change of immunoglobulin M by spin-labeling at the carbohydrate and peptide moieties of the molecule. Mol Biol (Mosk). 1982;16(2):403-10.
[3] Lapuk VA, Timofeev VP, Tchukchrova AI, Khatiashvili NM, Kiseleva TM. Some peculiarities of the dynamics of the immunoglobulin M structure. J Biomol Struct Dyn. 1984;2(1):63-76.
[4] Sharom FJ, Ross TE. Spin labelling of sialic acid and galactose residues on lymphocyte plasma membrane: effects of lectins on oligosaccharide dynamics. Mol Immunol. 1985;22(5):521-30.
[5] Putnam FW, Florent G, Paul C, Shinoda T, Shimizu A. Complete amino acid sequence of the Mu heavy chain of a human IgM immunoglobulin. Science. 1973;182(4109):287-91.
[6] Kaverzneva ED, Chukhrova AI, Vikha GV. The role of carbohydrate groups in immunoglobulins M. V. Steric accessibility of oligosaccharide groupings in macroimmunoglobulins. Russian Journal of Bioorganic Chemistry. 1978; 4(3):369-374.
[7] Lapuk VA, Shmakova FV, Kaverzneva ED. Role of carbohydrate groups in immunoglobolins M. I.Isolation and properties of human immunoglobulin M with carbohydrate groups bound to L-chains. Russian Journal of Bioorganic Chemistry. 1975; 1(8):1134-9
[8] Warren L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959;234(8):1971-5.
[9] Dudich IV, Timofeev VP, Vol'kenshtein MV, Misharin AIu. Macromolecule rotative correlation time measurement by ESR for covalently bound spin label. Mol Biol (Mosk). 1977;11(3):685-93.
[10] Timofeev VP, Dudich IV, Sykulev YK, Nezlin RS. Rotational correlation times of IgG and its fragments spin-labeled at carbohydrate or protein moieties. Spatially fixed position of the Fc carbohydrate. FEBS Lett. 1978;89(2):191-5.
[11] Timofeev VP, Dudich IV, Volkenstein MV. Comparative study of dynamic structure of pig and chicken aspartate aminotransferases by measuring the rotational correlation time. Biophys Struct Mech. 1980;7(1):41-9.
[12] Timofeev VP. Characterization of dynamic behavior of side groups of globular proteins by spin-label, NMR and luminescence polarization methods. Mol Biol (Mosk). 1983;17(3):519-31.