Biopolym. Cell. 1988; 4(1):48-53.
Gene-Engineering Biotechnology
Transformation of ltk aprt cells by DNA assembled in nucleoprotein complexes
1Zakharian R. A., 1Galstian G. G., 1Gevorkian N. R., 1Galstian M. G., 1Amirkhanova L. M.
  1. Institute of Experimental Biology, Academy of Sciences of the Armenian SSR
    Yerevan, USSR

Abstract

The nucleosome reconstitution was carried out in the presence of DNA, histones and anionic DNA-binding proteins of blood plasma, which serve as a stimulator of self-assembly process. The least sizes of DNA fragment protected from the micrococcal nuclease action corresponds to the DNA value from intact core nucleosome particle. Reconstructed complexes are biologically active in transformation of eukaryotic cells (Ltkaprt).

References

[1] Shows TB, Sakaguchi AY. Gene transfer and gene mapping in mammalian cells in culture. In Vitro. 1980;16(1):55-76.
[2] Bar-Eli M, Mercola KE, Slamon DJ, Mauritzson N, Stang HD, Cline MJ. Insertion of drug resistance genes in animals. J Cell Physiol Suppl. 1982;1:213-7.
[3] Slilaty SN, Aposhian HV. Gene transfer by polyoma-like particles assembled in a cell-free system. Science. 1983;220(4598):725-7.
[4] Zakharyan RA, Gasparyan ET, Aposhyan VG. Transport of β-lactamase gene into human cells by PLP and its expression. Biol Zh Armenii. 1982; 36(9):730-4.
[5] Pellicer A, Wigler M, Axel R, Silverstein S. The transfer and stable integration of the HSV thymidine kinase gene into mouse cells. Cell. 1978;14(1):133-41.
[6] Milman G, Herzberg M. Efficient DNA transfection and rapid assay for thymidine kinase activity and viral antigenic determinants. Somatic Cell Genet. 1981;7(2):161-70.
[7] Farber FE, Eberle R. Effects of cytochalasin and alkaloid drugs on the biological expression of herpes simplex virus type 2 DNA. Exp Cell Res. 1976;103(1):15-22.
[8] Miller CL, Ruddle FH. Co-transfer of human X-linked markers into murine somatic cells via isolated metaphase chromosomes. Proc Natl Acad Sci U S A. 1978;75(7):3346-50.
[9] Nicolau C, Soriano P, Lepape A, Londosgagliardi D, Legrand A, et al. Liposomes as carriers for gene transfer in vivo. Biol Cell. 1983; 47(1):121-129.
[10] Schaefer-Ridder M, Wang Y, Hofschneider PH. Liposomes as gene carriers: efficient transformation of mouse L cells by thymidine kinase gene. Science. 1982;215(4529):166-8.
[11] Bendich A, Wilczok T, Borenfreund E. Circulating DNA as a possible factor in oncogenesis. Science. 1965;148(3668):374-6.
[12] Brehm SP, Hoch SO, Hoch JA. DNA-binding proteins in human serum. Biochem Biophys Res Commun. 1975;63(1):24-31.
[13] Siddiqui AA, Davies RJ, Hill JA. The fractionation and isolation of DNA-binding proteins from human serum [proccedings]. Biochem Soc Trans. 1979;7(5):1088-9.
[14] Hemmaplardh D, Morgan EH. Transferrin uptake and release by reticulocytes treated with proteolytic enzymes and neuraminidase. Biochim Biophys Acta. 1976;426(3):385-98.
[15] Litman RM. A deoxyribonucleic acid polymerase from Micrococcus luteus (Micrococcus lysodeikticus) isolated on deoxyribonucleic acid-cellulose. J Biol Chem. 1968;243(23):6222-33.
[16] Bonne-Andrea C, Harper F, Sobczak J, De Recondo AM. Rat liver HMG1: a physiological nucleosome assembly factor. EMBO J. 1984;3(5):1193-9.
[17] Thomas JO, Butler PJ. Characterization of the octamer of histones free in solution. J Mol Biol. 1977;116(4):769-81.
[18] Libertini LJ, Small EW. Salt induced transitions of chromatin core particles studied by tyrosine fluorescence anisotropy. Nucleic Acids Res. 1980;8(16):3517-34.
[19] Lutter LC. Kinetic analysis of deoxyribonuclease I cleavages in the nucleosome core: evidence for a DNA superhelix. J Mol Biol. 1978;124(2):391-420.
[20] Hayashi K. Distribution of histone F1 on calf thymus nucleohistone DNA. J Mol Biol. 1975;94(3):397-408.
[21] Laskey RA, Honda BM, Mills AD, Finch JT. Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA. Nature. 1978;275(5679):416-20.
[22] Stein A, Whitlock JP Jr, Bina M. Acidic polypeptides can assemble both histones and chromatin in vitro at physiological ionic strength. Proc Natl Acad Sci U S A. 1979;76(10):5000-4.
[23] Ruiz-Carrillo A, Jorcano JL, Eder G, Lurz R. In vitro core particle and nucleosome assembly at physiological ionic strength. Proc Natl Acad Sci U S A. 1979;76(7):3284-8.
[24] Langmore JP, Wooley JC. Chromatin architecture: investigation of a subunit of chromatin by dark field electron microscopy. Proc Natl Acad Sci U S A. 1975;72(7):2691-5.
[25] Laskey RA, Earnshaw WC. Nucleosome assembly. Nature. 1980;286(5775):763-7.
[26] Nelson T, Wiegand R, Brutlag D. Ribonucleic acid and other polyanions facilitate chromatin assembly in vitro. Biochemistry. 1981;20(9):2594-601.
[27] Wigler M, Pellicer A, Silverstein S, Axel R, Urlaub G, Chasin L. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells. Proc Natl Acad Sci U S A. 1979;76(3):1373-6.