Biopolym. Cell. 2020; 36(3):182-196.
Обзоры
Системы трехмерного культивирования клеток
1, 2Сукач А. Н., 1Шевченко М. В.
  1. Институт проблем криобиологии и криомедицины НАН Украины
    23, Переяславская ул., Харьков, Украина, 61015
  2. Харьковский национальный педагогический университет имени Г. С. Сковороды
    ул. Алчевских, 29, Харьков, Украина, 61002

Abstract

В обзоре обсуждаются особенности трехмерных систем культивирования клеток на носителях (скаффолдах) и без носителей. Скаффолды используют с целью имитации внеклеточного матрикса, а также для воссоздания естественного физического и структурного микроокружения клеток, подобного живой ткани. В обзоре рассматриваются типы скаффолдов (жесткие и гелеобразные, природные и искусственные, разлагаемые и не разлагаемые), их характеристики, преимущества, недостатки и особенности заселения клетками. Обсуждается использование в качестве скаффолдов децеллюлялизированных и девитализированных органов и тканей. Также в обзоре рассматривается безматриксное культивирование клеток в составе трехмерных многоклеточных структур – сфероидов. Обсуждается структура и биология сфероидов. Рассматриваются особенности образования сфероидов в статических (самосборка) и динамических (под воздействием внешних сил) условиях культивирования. Обсуждается роль размера сфероидов для выживания клеток.
Keywords: клетки, 3D культура клеток, скаффолды, сфероиды

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