Biopolym. Cell. 2019; 35(5):340-348.
Молекулярная и клеточная биотехнологии
Nicotiana cavicola как хозяин для производства биофармацевтических препаратов в растениях путём Agrobacterium-опосредованной транзиентной экспрессии генов
1Синдаровская Я. Р., 2Олевинская З. М., 2Демченко А. А., 2Спивак Н. Я., 1Кучук Н. В.
  1. Институт клеточной биологии и генетической инженерии НАН Украины
    ул. Академика Заболотного, 148, Киев, Украина, 03143
  2. Институт микробиологии и вирусологии им. Д. К. Заболотного НАН Украины
    ул. Академика Заболотного, 154, Киев, Украина, 03143

Abstract

Цель. Анализировать новый вид растений в качестве хозяина для получения рекомбинантных белков посредством транзиентной экспрессии генов. Методы. Agrobacterium-опосредованная транзиентная экспрессия генов, анализ белков, статистическая обработка данных. Результаты. Растения N. cavicola демонстрируют хорошие биотехнологические характеристики, и они восприимчивы к агробактериальной инфекции и к растительным вирусам. В результате транзиентной экспрессии генов с использованием двух различных векторных систем в N. cavicola нарабатывался зеленый флуоресцентный белок (GFP) и человеческий интерферон альфа. Уровень рекомбинантных белков зависел от используемого гена и системы. Содержание GFP достигало 6,0 % и 12,6 % СРБ (0,44 мг/г СВ). Противовирусная активность интерферона из экстрактов листьев составляла 840 МЕ/г СВ и 1710 МЕ/г СВ. Выводы. В данной статье мы предлагаем вид N. cavicola в качестве нового хозяина для получения рекомбинантных белков, который может быть использован как альтернатива хозяину N. benthamiana.
Keywords: Nicotiana cavicola, Agrobacterium-опосредованная транзиентная экспрессия, рекомбинантные белки, человеческий интерферон альфа, GFP

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