Biopolym. Cell. 2019; 35(3):169-169.
Хроника и информация
TPR, chromatin organization, gene expression and cell development
1, 4Ухрилова Ж., 1, 4Фишерова Ж., 2Фишер К., 1, 3, 4Хозак П.
  1. Department of Biology of the Cell Nucleus, Institute of Molecular Genetics CAS, v.v.i.,
    Vídeňská 1083, Prague, Czech Republic
  2. CLIP-Childhood Leukaemia Investigation Prague, Department of Pediatric Hematology/Oncology; Medical Faculty of the Charles University and University Hospital Motol,
    V Úvalu 84, Prague, Czech Republic
  3. Microscopy Centre – LM and EM, Institute of Molecular Genetics CAS, v.v.i.,
    Vídeňská 1083, Prague, Czech Republic;
  4. Division BIOCEV, Institute of Molecular Genetics CAS, v.v.i.,
    Průmyslová 595, Vestec, Prague, Czech Republic

Abstract

The area around nuclear pores is enriched in open chromatin. Transcriptional factors and super enhancer sequences localize there during cell differentiation, activating sets of genes specific for a given tissue. Nucleoporin TPR is crucial for the enrichment of open chromatin around nuclear pores, however its role in gene expression and cell development has not been described yet. Here we show that depletion of TPR results in aberrant morphology of murine proliferating C2C12 myoblasts (MB) and differentiated C2C12 myotubes (MT). Our CHIP-seq data revealed that TPR binds to Myosin heavy chain (Myh) gene and majority of olfactory receptor (Olfr) genes in C2C12 MB, and its binding pattern changes upon differentiation into MT. Both Myh and Olfr are expressed in muscle cell and regulate the muscle formation and morphology. We show that TPR affects expression of both Myh and Olfr genes, however in a different manner. Here we discuss possible pathways by which TPR regulates expression of bound genes. This work was supported by the GACR (15-08835Y, 16-03403S, 18-19714S), GAUK (930218) and by the institutional support (RVO: 68378050). I acknowledge the Microscopy Centre funded by MEYS CR (LM2015062, Czech-BioImaging) for their support.