Biopolym. Cell. 2019; 35(1):16-20.
http://dx.doi.org/10.7124/bc.000996
Structure and Function of Biopolymers
Aggregation of erythrocytes: a novel activity of HBD-2
1Semeniuk D. O., 1Starenka I. O., 2Pogribna A. P., 3Sokol I. V., 3Govsieiev D. O.
  1. Educational and Scientific Center "Institute of Biology and Medicine",
    Taras Shevchenko National University of Kyiv
    64/13, Volodymyrska Str., Kyiv, Ukraine, 01601
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143
  3. Kyiv city maternity hospital N 5
    2, Lobanovskiy Ave., Kyiv, Ukraine, 02000

Abstract

Aim. To investigate an ability of HBD-2 to induce aggregation of erythrocytes. Methods. Aggregation assay, turbidimetry, light microscopy. Results. We have identified aggregation of erythrocytes in presence of at least 12.5 μM of HBD-2 that was confirmed by light microscopy. Effect was depended on HBD-2 concentration and diminished in presence of NaCl. Conclusions. HBD-2 can induce salt-sensitive aggregation of erythrocytes in dose-dependent manner.
Keywords: β-defenins, dose-dependent, salt-sensitive
Full text: (PDF, in English)

References

[1] Bals R, Wang X, Wu Z, Freeman T, Bafna V, Zasloff M, Wilson JM. Human beta-defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung. J Clin Invest. 1998;102(5):874-80.
[2] Chen RB, Zhang K, Zhang H, Gao CY, Li CL. Analysis of the antimicrobial mechanism of porcine beta defensin 2 against E. coli by electron microscopy and differentially expressed genes. Sci Rep. 2018;8(1):14711.
[3] Semple F, Dorin JR. β-Defensins: multifunctional modulators of infection, inflammation and more? J Innate Immun. 2012;4(4):337-48.
[4] Wimley WC, Selsted ME, White SH. Interactions between human defensins and lipid bilayers: evidence for formation of multimeric pores. Protein Sci. 1994;3(9):1362-73.
[5] Cummings JE, Vanderlick TK. Aggregation and hemi-fusion of anionic vesicles induced by the antimicrobial peptide cryptdin-4. Biochim Biophys Acta. 2007;1768(7):1796-804.
[6] Pogribnoy PV, Lisovskiy IL, Markeeva NV, Shnitsar VM, Zinchenko II, Soldatkina MA. Production of recombinant of hbd-2 — human antimicrobial peptide expressed in cervical and vulval cancer. Exp Oncol. 2003; 25(1):36-39.
[7] Makarenko MV, Semeniuk DO, Starenka IO, Pogribna AP, Sokol IV, Martinova LI, Govsieiev DO. HBD-2 interactions with erythrocyte membranes in vitro. Ukr Biochem J. 2019; 91(1):65-72.