Biopolym. Cell. 2017; 33(3):206-213.
Биоинформатика
Сходство и отличие первичных структур геномов ряда стрептомицетов и хромосомной ДНК Streptomyces globisporus 1912-2
1Полищук Л. В.
  1. Институт микробиологии и вирусологии им. Д. К. Заболотного НАН Украины
    ул. Академика Заболотного, 154, Киев, Украина, 03680

Abstract

Цель. Определить in silico сходство и отличие первичных структур геномов ряда штаммов стрептомицетов и хромосомной ДНК S. globisporus 1912-2. Методы. Использовали ресурсы сервера NCBI (BLAST: blastn и bl2seq: megablast) для in silico анализа первичной структуры контигов S. globisporus 1912‑2 и ряда Интернет баз данных NCBI (Genome, Nucleotide). Результаты. Выявлено ряд штаммов со значительными показателями гомологии их первичной структуры ДНК и нуклеотидной последовательности хромосомной ДНК S. globisporus 1912-2 (степенью идентичности (88–97 %) и степенью покрытием (55–82 %)). Максимальная идентичность нуклеотидной последовательности хромосомной ДНК S. globisporus 1912‑2 была выявлена у геномов штаммов S. globisporus C-1027 (97 %) и S. griseus NBRC13350 (96 %). Ни одного фрагмента с первичной структурой, гомологичной структурам семи контигов S. globisporus 1912‑2 не было найдено среди нуклеотидных последовательностей стрептомицетов из базы данных NCBI. Выводы. Установлено, что штамм S. globisporus 1912‑2 является членом клады S. griseus. Мы выявили большой биосинтетический потенциал штамма S. globisporus 1912-2, возможный благодаря его многим уникальным нуклеотидным последовательностям.
Keywords: стрептомицет, первичная струк-тура, геном, идентичность, in silico анализ, клада.

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