Biopolym. Cell. 1986; 2(1):50-52.
http://dx.doi.org/10.7124/bc.00019B
Short Communications
Detachment of DNA from the nuclear matrix in growth-arrested transformed chinese hamster fibroblasts
1Sjakste N. I., 1Sjakste T. G.
  1. Research Institute of Experimental and Clinical Medicine, Ministry of Public Health, Latvian SSR
    Riga, USSR

Abstract

SV-40 transformed Chinese hamster fibroblasts were growth-arrested by incubation in the medium with lowered serum content. DNA-protein interactions in the cells were studied by the nucleoprotein-celite-chromatography method. Detachment of the DNA from the nuclear matrix was revealed in quiescent cells. After the cells were restimulated to proliferate the reassociation of DNA and nuclear matrix was observed. The weakening of the bonds of a part of DNA with nuclear matrix proteins was also observed in quiescent fibroblasts.
Full text: (PDF, in Russian)

References

[1] S'iakste NI, Zaboikin MM, Lichtenstein AV, Shapot VS. Analysis of cellular nucleoproteins by nucleoprotein-celite-chromatography. I. Structural rearrangements of the chromosomal apparatus coupled with cellular quiescence-growth transitions. Mol Biol (Mosk). 1981;15(6):1321-9.
[2] Rearrangements of DNA - protein interactions in animal cells coupled with cellular growth-quiescence transitions . A. V. Lichtenstein, N. I. Sjakste, M. M. Zaboykin, V. S. Shapot. Nucl. Acids Res. 1982. 10, N 3:1127-1145.
[3] S'iakste NI, Zaboikin MM, Erenpreisa EA, Likhtenshtein AV, Shapot VS. Analysis of cellular nucleoproteins by the nucleoprotein-celite chromatography method. III. Two types of DNA-nuclear matrix interaction. Mol Biol (Mosk). 1985;19(5):1231-41.
[4] Kakpakova ES, Sokova OI, Levina NV. Biological and karyological characteristics of Djungarian hamster cell lines transformed by SV-40 virus. Tsitologiia. 1972;14(8):1019-26.
[5] Likhtenshtein AV, Gradient dissociation of total cell nucleoproteins as a principle of separation and analysis of functionally different types of nucleic acids. 245, no. 1. 6, 3 April 1979( XP000569286 ISSN: 0012-4958) pages 106-109.
[6] Zbarskiy IB. Structural organization and functional role of nuclear matrix. Structural and functional aspects of DNA replication and repair. Pushchino, 1983; 3-21.
[7] Dubrow R, Riddle VG, Pardee AB. Different responses to drugs and serum of cells transformed by various means. Cancer Res. 1979;39(7 Pt 1):2718-26.