Biopolym. Cell. 2003; 19(6):530-535.
http://dx.doi.org/10.7124/bc.00067F
Molecular and Cell Biotechnologies
Influence of various glycerol concentrations on the yield and solubility of the Escherichia coli recombinant methionine aminopeptidase
1, 2Slavchenko I. Yu., 1Boreyko E. V., 1Vorobey N. V.
  1. PSRS "Biotechnolog"
    150, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The influence of glycerol on the yield and solubility of a recombinant protein in a model system of E. coli methionine aminopeptidase (MAP) overproduction using the bacteriophage 77 RNA polymerase has been investigated. We have found, that glycerol added in a rich medium may inhibit the producer growth, promote the MAP accumulation in a soluble form and influence the target product yield. It has been shown, that manipulation of such parameters as glycerol concentration and culture optical density, when glycerol is added to the medium, allows purposely to increase the MAP yield in a soluble form. The approaches developed may be applicable for the production of other recombinant proteins in E. coli cells.
Full text: (PDF, in Russian)

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