Biopolym. Cell. 2002; 18(6):485-488.
Structure and Function of Biopolymers
Restriction polymorphism of amplified fragments of the genus Nicotiana chloroplast DNA
1Komarnytskyy S. I., 1Komarnytskyy I. K.
  1. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

Using the PCR amplification of short regions of the chloroplast DNA together with their subsequent restriction digestion, it is possible to follow the cytoplasm inheritance during the genus Nicotiana development. The data obtained confirmed the cultivated tobacco cytoplasm to originate from the Rustica subgenus ancestors. Two Australian species, N. debneyi and N. fragrans, have nothing in common with, the cytoplasm of other species of Suaveolentes section of the genus.

References

[1] Goodspeed TH. The genus Niconiana. Massachusetts: Waltham, 1954. 536 p.
[2] Kostoff D. Cytogenetics of the genus Nicotiana. Sofia: State Printed House, 1941;43: 1071 p.
[3] Kung SD, Zhu YS, Shen GF. Nicotiana chloroplast genome III. Chloroplast DNA evolution. Theor Appl Genet. 1982;61(1):73-9.
[4] Komarnyts'ky? SI, Komarnyts'ky? IK, Cox A, Parokonny? AS. [The evolution of the sequences of the internal spacer of nuclear ribosomal DNA for American species in the genus Nicotiana]. Tsitol Genet. 1998;32(3):69-76.
[5] Komarnitsky SI, Komarnistky IK, Cox A, Parokonny AS. [Molecular phylogeny of the nuclear 5.8S ribosomal RNA genes in 37 species of Nicotiana genus]. Genetika. 1998;34(7):883-9. Russian.
[6] Komarnytsky SI. An attempt to combine morphological characters and nuclear ribosomal DNA (internal transcribed spacer) sequences in phylogenetic studies in the genus Nicotiana. Biopolym Cell. 1999; 15(5):383-9.
[7] Parducci L, Szmidt AE. PCR-RFLP analysis of cpDNA in the genus Abies. Theor Appl Genet. 1999;98(5):802–8.
[8] Tsumura Y, Kawahara T, Wickneswari R, Yoshimura K. Molecular phylogeny of Dipterocarpaceae in Southeast Asia using RFLP of PCR-amplified chloroplast genes. Theor Appl Genet. 1996;93(1-2):22-9.
[9] Parani M, Lakshmi M, Ziegenhagen B, Fladung M, Senthilkumar P, Parida A. Molecular phylogeny of mangroves VII. PCR-RFLP of trn S- psb C and rbc L gene regions in 24 mangrove and mangrove-associate species. Theor Appl Genet. 2000;100(3-4):454–60.
[10] Cheung WY, Hubert N, Landry BS. A simple and rapid DNA microextraction method for plant, animal, and insect suitable for RAPD and other PCR analyses. PCR Methods Appl. 1993;3(1):69-70.
[11] Savolainen V, Corbaz R, Moncousin C, Spichiger R, Manen JF. Chloroplast DNA variation and parentage analysis in 55 apples. Theor Appl Genet. 1995;90(7-8):1138-41.
[12] Taberlet P, Gielly L, Pautou G, Bouvet J. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Biol. 1991;17(5):1105-9.
[13] Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayshida N, Matsubayasha T, et al. The complete nucleotide sequence of the tobacco chloroplast genome. Plant Mol Biol Rep. 1986;4(3):111–48.
[14] Komarnitskii IK, Cherep NN. Restriction fragment length polymorphism of mitochondrial DNA of the genus Nicotiana. Tsitol Genet. 1994; 28(4):47-59.
[15] Salts Y, Herrmann RG, Peleg N, Lavi U, Izhar S, Frankel R, Beckmann JS. Physical mapping of plastid DNA variation among eleven Nicotiana species. Theor Appl Genet. 1984;69(1):1-14.
[16] Komarnytsky SI. Subrepeats of the intergenic spacer of the Nicotiana ribosomal DNA. Biopolym Cell. 2000; 16(2):108-14.
[17] Gray JC, Kung SD, Wildman SG, Sheen SJ. Origin of Nicotiana tabacum L. detected by polypeptide composition of Fraction I protein. Nature. 1974;252(5480):226–7.