Biopolym. Cell. 2002; 18(2):164-170.
Молекулярная и клеточная биотехнологии
Изучение особенностей синтеза альфа-2b интерферона человека в клетках Escherichia coli при различных условиях культивирования
1Славченко И. Ю.
  1. ПНИК «Биотехнолог»
    ул. Академика Заболотного, 150, Киев, Украина, 03680

Abstract

Исследовано влияние на уровень синтеза альфа-2b интерферона (ИФН) человека в клетках Е. coli SG30 (pIF-16) наличия в питательной среде глюкозы и мальтозы, а также температуры культивирования продуцента. Продуцент ИФН содержит плазмиду pIF-16, несущую тандем искусственных генов ИНФ. Конститутивную экспрессию этих генов обеспечивает тандем триптофановых промоторов. В результате исследований выявлено, что при выращивании клеток Е. coli SG30 (pIF-16) при температуре 37 °С в среде, содержащей в качестве источников углерода мальтозу или глюкозу, может существенно снижаться уровень выхода ИФН по сравнению со средой без добавления этих сахаров. Показано, что выход ИФН зависит от температуры культивирования продуцента и концентрации данных сахаров в питательной среде. Глюкоза оказывает негативное влияние на синтез ИФН в более низких концентрациях, чем мальтоза. Установлено, что добавление мальтозы в культуральную среду обеспечивает более высокий выход биомассы, чем добавление глюкозы, при выращивании клеток штамма-продуцента Е. coli SG30 (plF-lb) как при температуре 37, так и 28 °С.

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