Biopolym. Cell. 2002; 18(2):135-138.
Разработка метода массового отбора мутантов с поврежденной инактивацией пероксисомных ферментов дрожжей Yarrowia lipolytica
1Назарко Т. Ю., 1Мала М. Е., 1Сибирный А. А.
  1. Институт биологии клетки НАН Украины
    ул. Драгоманова, 14/16, Львов, Украина, 79005

Abstract

Разработан метод массового отбора мутантов дрожжей Y. lipolytica с поврежденной инактивацией пероксисомных ферментов. Получены первые мутанты с блоком инактивации пероксисомных аминооксидазы и изоцитратлиазы путем инсерции кассеты zeta-URA3 в геном свободного от zeta-елементов делеционного мутанта ura3 Y. lipolytica.

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