Biopolym. Cell. 1999; 15(5):390-394.
http://dx.doi.org/10.7124/bc.000535
Structure and Function of Biopolymers
Influence of antioxidants on catalytic activity of the respiratory chain of mitochondria electronic transport in rat liver at paracetamol intoxication
1Blazhchuk I. S., 1Kovalenko V. N., 1Shayakhmetova A. M., 1Voronina A. K., 2Kuzmenko I. V.
  1. Institute of Pharmacology and Toxicology of National Medical Academy of Science of Ukraine
    14, Eugene Pottier Str., Kyiv, Ukraine, 03057
  2. Palladin Institute of Biochemistry, NAS of Ukraine
    9, Leontovycha Str., Kyiv, Ukraine, 01601

Abstract

Over 2-fold increase in superoxide anion and hydroperoxide level was revealed in tat liver mitochondria at acute paracetamol intoxication. It is accompanied with about 35 % decrease in both NAD-and succinate-cytochrom-c oxidoreductase activities. Pcroral administration of two α-tocoferol acetate derivatives with different length of a isoprenoid chain promotes an increase in antioxidant status of mitochondria and reduction of the activity of the enzyme systems independently on the structure of the side chain of vitamin E analogs.
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References

[1] Hinson JA, Pumford NR, Roberts DW. Mechanisms of acetaminophen toxicity: immunochemical detection of drug-protein adducts. Drug Metab Rev. 1995;27(1-2):73-92.
[2] Manautou JE, Chen C., McCann D.J. In vivo covalent binding of acetaminophen to canalicular and basolateral membrane proteins in mouse liver. Toxicol Lett, 1998; 95:161.
[3] Miner DJ, Kissinger PT. Evidence for the involvement of N-acetyl-p- quinoneimine in acetaminophen metabolism. Biochem Pharmacol. 1979;28(22):3285-90.
[4] Moore M, Thor H, Moore G, Nelson S, Mold?us P, Orrenius S. The toxicity of acetaminophen and N-acetyl-p-benzoquinone imine in isolated hepatocytes is associated with thiol depletion and increased cytosolic Ca2+. J Biol Chem. 1985;260(24):13035-40.
[5] Lo-Quoc K, Le-Quoc D. Control of the mitochondrial inner membrane permeability by sulfhydryl groups. Arch Biochem Biophys. 1982;216(2):639-51.
[6] Burcham PC, Harman AW. Effect of acetaminophen hepatotoxicity on hepatic mitochondrial and microsomal calcium contents in mice. Toxicol Lett. 1988;44(1-2):91-9.
[7] Korneev AA, Komissarova IA. Adaptive responses of respiratory chain: molecular mechanisms of formation. Usp Sovrem Biol. 1994; 114(4):467-74.
[8] Manuylov V. G., Ivanova V. F., Puzyrev A. A. structural homeostasis by the action of some anthropogenic factors Hygiene and sanitation. 1994; (1) :30-32.
[9] Adamson GM, Harman AW. A role for the glutathione peroxidase/reductase enzyme system in the protection from paracetamol toxicity in isolated mouse hepatocytes. Biochem Pharmacol. 1989;38(19):3323-30.
[10] Kyle ME, Miccadei S, Nakae D, Farber JL. Superoxide dismutase and catalase protect cultured hepatocytes from the cytotoxicity of acetaminophen. Biochem Biophys Res Commun. 1987;149(3):889-96.
[11] Harman AW, Adamson GM, Shaw SG. Protection from oxidative damage in mouse liver cells. Toxicol Lett. 1992;64-65 Spec No:581-7.
[12] Farber JL, Kyle ME, Coleman JB. Mechanisms of cell injury by activated oxygen species. Lab Invest. 1990;62(6):670-9.
[13] Trufanov L. Biochemistry vitamins and antivitamin. M.: Kolos, 1972, 328.
[14] Hashimova MR, Imamova EA. Some indicators liver metabolism by the action of different doses of vitamin E. Zdorov'e Tadzhikistana. 1989; 4:92-94.
[15] Shneider V.S. Intracellular distribution of enzymes. III. The oxidation of octanoic acid by rat liver fractione. J. Biol. Chem. 1948; 176:259-262.
[16] Tisdale, H.D. [39] Preparation and properties of succinic-cytochrome c reductase (complex II-III) (1967) Methods in Enzymology, 10 (C), pp. 213-215.
[17] Hatefi Y., Rieske J.S. [41] The preparation and properties of DPNH-cytochrome c reductase (complex I-III of the respiratory chain). Methods in Enzymology, 1967; 10 (C):225-231.
[18] Babior BM, Kipnes RS, Curnutte JT. Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent. J Clin Invest. 1973;52(3):741-4.
[19] Romanova L. A., Stal'naya I. D. Method of determination lipid hydroperoxides using ammonium thiocyanate. Modern methods in biology, ed. VN Orekhovich M., 1977. 64-66.
[20] Stal'naya I. D., Garishvili T. G. Method of determining malondialdehyde using thiobarbituric acid. Modern Methods in Biology, ed. VN Orekhovich. M., 1977 44-46.
[21] Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265-75.
[22] Burcham PC, Harman AW. Acetaminophen toxicity results in site-specific mitochondrial damage in isolated mouse hepatocytes. J Biol Chem. 1991;266(8):5049-54.
[23] Torchinskiy Yu. Sulphur in Proteins. M.: Nauka, 1977. 302
[24] Vengerovskii AI, Saratikov AS. The mechanisms of the hepatotoxicity of paracetamol. Farmakol Toksikol. 1991;54(1):76-80.
[25] Kovalenko VN, Blazhchuk IS, Shaiakhmetova AM, Kuz'menko IV. Correction of the activity of certain enzymes in the rat liver mitochondrial electron transport chain by derivatives of alpha-tocopheryl acetate in toxic damage to the liver. Ukr Biokhim Zh. 1999; 71(4):30-4.
[26] Tlwmsen M.S. Oxidative metabolism of acetaminophen (paracetamol) to a reactive species: Envolved cytochrome P 450 enzymes and target toxicity related to covalent binding. Ugeskr. Leager, 1996; 158 (28):4095-4096.
[27] Poskrypko A. antiradical and antioxidant activity of structurally modified analogues of ?-tocopherol. Liky. 1998; 2:76-80.
[28] Manoylov S. E., Sedykh V. On the Mechanism succinate oxidation in the liver of albino rats. Bull Exp Biol Med. 1996; 5:524-525.
[29] Semenov VL, Iarosh AM. The effect of hypoxia on oxidative phosphorylation and lipid peroxidation in rat liver mitochondria upon lung inflammation. Ukr Biokhim Zh. 1991;63(2):95-101.