Biopolym. Cell. 1999; 15(4):306-313.
Viruses and Cell
Investigation of mode of inactivating effect on tabacco mosaic vims of the cellular components from sugar beet leaves
1Kolesnik L. V.
  1. D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
    154, Academika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

Investigation of the chemical nature and mode of action of the antiviral component (AVC) isolated from cellular juice of sugar beet leaves by ion-exchange chromatography have been conducted. It was established that inactivating ability for AVC is caused by effect of low-molecular weight proteins with proteolytic activity related to structural protein of TMV binding to RNA, givins rise to RNP-complex. Inactivation of TMV is a result of A VC-absorption on the viral particles surface, violation of morphology, aggregation of virus, and restriction of plant infection efficiency. Inhibition of trans-criptional activity of viral RNA and protein synthesis, cytophysiological reconstruction into TMV infected cells, probably, limit the replication and systemic transport of virus, that is displyed in symptomless TMV-infection of sugar beet plants.

References

[1] Moraes W.B.C., July J.R., Alba A.P.C., Oliveira A.R. The inhibitory activity of extracts of Abutilon striatum leaves on plant virus infection. II. Mechanism on inhibition. Phytopathol Z., 1974; 81(3):240-253.
[2] Baranwal VK, Verma HN. Characteristics of a virus inhibitor from the leaf extract of Celosia cristata. Plant Pathology, 1997; 46(4):523-529.
[3] Smookler MM. Properties of inhibitors of plants virus infection occuring in the leaves of species in the Chenopodiales. Ann. Appl. Biol., 1971;69(2):157-168.
[4] Ito Y, Keki I, Tanifuji S, Hiramatsu A. Inhibition of protein synthesis by antiviral protein from Yucca recurfolia leaves. Biosci., Biotechnol. and Biochem., 1993; 57(3):518-519.
[5] Kolesnik LV. Peculiarities of tobacco mosaic virus-induced infection in sugar beet plants. Mikrobiol Zh. 1992; 54(4):58-66.
[6] Kolesnik LV, Kraeva GV. Isolation and characteristic of tobacco mosaic virus inhibitor from sugar beet leaves. Mikrobiol Zh. 1992; 54(6):58-63.
[7] Kolesnik LV. Effect of trypsin and an inhibitor of sugar beet leaves on infectivity and morphology of tobacco mosaic virus particles. Mikrobiol Zh. 1993; 55(5):31-7.
[8] Kolesnik LV. Studies on nature and mode of action of antiviral component from sugar beet leaves. Abstr. Int. Conf. Fundamental and applied problems in phytopathology., Yalta 1994:20.
[9] Troitsky GV, Azhitsky GYu. Borate-polyol pH gradient. A theoretical basis and application for protein isoelectrofocusing. Russian Journal of Bioorganic Chemistry 1982, 8 (6):863-871
[10] Clemens MJ. Translation of eukaryotic messenger RNA in cell-free extracts. In Transcription and Translation: A Practical Approach Ed. B. D. Hames (Editor), S. J. Higgins Oxford University Press 1984 231–70.
[11] Solohub LI, Pashkovs'ka IS, Antonyak HL. Cell's proteases and their functions. Kyyiv: Nauk, dumka, 1992. 196p.
[12] Dobroye Ye. Isolation of the structural protein of TMV acetate method. Praktikum po obshchey virusologii. Ed. I. G. Atabekova. M.: Izd-vo Mosk. un-ta, 1981; p111.
[13] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970; 227(5259):680-685.
[14] Davis BJ. Disc electrophoresis. ii. method and application to human serum Ann N Y Acad Sci. 1964;121:404-27.
[15] Ahl P, Cornu A, Gianinazzi S. Soluble proteins as genetic markers in studies of resistance and phylogeny in Nicotiana. Phytopathology., 1982; 22(1):80-85.
[16] Martins de Sa C, Grossi de Sa MF, Akhayat O, Broders F, Scherrer K, Horsch A, Schmid HP. Prosomes. Ubiquity and inter-species structural variation. J Mol Biol. 1986;187(4):479-93.
[17] Kremp A, Schliephacke M, Kull U, Schmid HP. Prosomes exist in plant cells too. Exp Cell Res. 1986;166(2):553-7.
[18] Parkhomenko NI, Didenko LF, Maximenko LA. Physicochemical properties of prosomes from leaves Datura stramonium L infected potato virus X. Biopolym. Cell. 1996; 12(6):102-111
[19] Schmid H-P. Prosomes associated with repressed globin mRNA inhibit protein synthesis in vitro. Eur. J. Cell Biol. 1985; Suppl., 36 (7):58.
[20] Horsch A, Kohler K, Schmid HP. Prosomes are involved in the repression of viral mRNA. Z Naturforsch C. 1985;40(5-6):449-50.
[21] Horsch A, Martins de Sa C, Dineva B, Spindler E, Schmid HP. Prosomes discriminate between mRNA of adenovirus-infected and uninfected HeLa cells. FEBS Lett. 1989;246(1-2):131-6.
[22] Horsch A, Kohler K, Ellwart-Tsch?rz M, Schmid HP. Selection of prosomes and prosomal RNA by immobilized viral RNAs. FEBS Lett. 1990;269(2):336-40.