Biopolym. Cell. 1999; 15(3):220-226.
Structure and Function of Biopolymers
The different factors influence on the serum immunoglobulms transformation into polyreactive immunoglobulins and their interaction with antigens
1Bobrovnik S. A., 1Chornaya V. E., 1Nikolaenko I. V.
  1. Palladin Institute of Biochemistry, NAS of Ukraine
    9, Leontovycha Str., Kyiv, Ukraine, 01601

Abstract

The optimal conditions were studied for the process of serum immunoglobulins activity increasing using sharp shift of pH or their incubation with chaotropic ions. It was shown that this process strongly depends on the temperature of incubation. The influence of pH and concentration of some salts on binding of polyreactive immunoglobulins (PRIG) to plate immobilised antigens were studied. The data obtained show that electrostatic forces play an important role in the PRIG-Ag interaction. It was also shown, that no: only serum immunoglobulins, but also highspecific monoclonal antibodies could be transformed into PRIG using high or low pH, or ctaoiropic ions.

References

[1] Bobrovnik SA. Activation of "silent" antibodies and their interaction with antigens. Ukr Biokhim Zh. 1990;62(5):86-9.
[2] Bobrovnik SL, Lyashchenko KP, Komissarenko SV. Polyspecific antibodies and thier activation. Doklady Akad Nauk Ukr SSR. Ser B. 1990; (6):71-4.
[3] Bobrovnik SA, Petrova IuI, Efetov KA. Transformation of serum immunoglobulins to polyreactive antibodies. Ukr Biokhim Zh. 1997;69(3):36-42.
[4] Bobrovnik SA. Transformation of serum immunoglobulins and monoclonal antibodies into polyreactive immunoglobulins. Ukr Biokhim Zh. 1997;69(5-6):97-109.
[5] Roggenbuck D, Konig H, Niemann B, Schoenherr G, Jahn S, Porstmann T. Real-time biospecific interaction analysis of a natural human polyreactive monoclonal IgM antibody and its Fab and scFv fragments with several antigens. Scand J Immunol. 1994;40(1):64-70.
[6] Labrousse H, Adib-Conquy M, Avrameas S. Effects of pH or ionic strength on the reactivities of mono- and polyspecific IgG antibodies. Res Immunol. 1994;145(7):541-52.
[7] Bobrovnik SA, Marinets AV. Properties of polyreactive immunoglobulins. Ukr Biokhim Zh. 1993;65(5):21-6.
[8] Shyrobokov VP, Nikolaienko IV, Kopanytsia LV, Solohub VK, Ivanova OIe. A monoclonal antibody panel for the intratypic differentiation of type-2 polioviruses. Mikrobiol Z. 1997;59(6):27-36.
[9] Bobrovnik SA, Starodub NF. New simple way to immobilize antigens on immunological plates. Immunologiya. 1988; 5:83—85.
[10] Hudson L, Hay FC. Practical immunology. Oxford: Alden press, 1976. 298 p.
[11] Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.
[12] Dixon M., E. Webb Enzymes. M.: Mir, 1982. vol 1, 389 p.
[13] Casali P, Notkins AL. Probing the human B-cell repertoire with EBV: polyreactive antibodies and CD5+ B lymphocytes. Annu Rev Immunol. 1989;7:513-35.
[14] Avrameas S. Natural autoantibodies: from 'horror autotoxicus' to 'gnothi seauton'. Immunol Today. 1991;12(5):154-9.
[15] Avrameas S, Ternynck T. The natural autoantibodies system: between hypotheses and facts. Mol Immunol. 1993;30(12):1133-42.
[16] Ruoslahti E. Antigen-Antibody Interaction, Antibody Affinity, and Dissociation of Immune Complexes. Scand J Immunol. 1976; 3:3—7.
[17] Sigounas G, Kolaitis N, Monell-Torrens E, Notkins AL. Polyreactive IgM antibodies in the circulation are masked by antigen binding. J Clin Immunol. 1994;14(6):375-81.
[18] Absolom DR, van Oss CJ. The nature of the antigen-antibody bond and the factors affecting its association and dissociation. CRC Crit Rev Immunol. 1986;6(1):1-46.
[19] Padlan EA. Anatomy of the antibody molecule. Mol Immunol. 1994;31(3):169-217.
[20] Gonzalez R, Matsiota P, Torchy C, De Kinkelin P, Avrameas S. Natural anti-TNP antibodies from rainbow trout interfere with viral infection in vitro. Res Immunol. 1989;140(7):675-84.
[21] Coutinho A. Beyond clonal selection and network. Immunol Rev. 1989;110:63-87.
[22] Coutinho A, Avrameas S. Speculations on immunosomatics: potential diagnostic and therapeutic value of immune homeostasis concepts. Scand J Immunol. 1992;36(4):527-32.