Biopolym. Cell. 1997; 13(4):269-273.
http://dx.doi.org/10.7124/bc.000489
Structure and Function of Biopolymers
The aclion of vitamin E on RNA- and DNA-polymerase activity of rat liver mitochondria
1Kapralov A. A.
  1. Petr Bogach Institute of Physiology
    Taras Shevchenko National University of Kyiv
    2, Academika Glushkova Ave Str., Kyiv, Ukraine, 03187

Abstract

The action of tocopherol on RNA- and DNA-polymerase activity of isolated rat liver mitochondria in condition of its joined addition with Ca2+ -ionophor A23187, Ca2+-channel blacker verapamil or protein kinase C inhibitor pliorbol I2-myristate-13-acetate (PMA) were studied. It was found, that tocopherol, A23187 and verapamil effected RNA and DNA synthesis in mitochondria only if added together with fraction of tocopherol-binding proteins. In case of addition of this fraction together with tocopherol and A23J87 or verapamil, the RNA-polymerase activity of mitochondria of normal rats increased compared to assayes in which only tocopherol and tocoplierot-binding proteins were added. Unlike this, joint addition of tocopherol binding protein, A23187 and verapamil caused inhibition of DNA synthesis in mitochondria of normal but not vitamin E-deficient ruts. The addition of tocopherol decreased the action of these compounds. There were found the differences between RNA-polymerase activity of normal and vitamin E-deficient rats in presence of PMA. It was suggested that Ca ions and protein kinase C may be involved in action of vitamin E on RNA synthesis in rat liver mitochondria.
Full text: (PDF, in Russian)

References

[1] Kapralov AA, Petrova GV, Donchenko GV. Physico-chemical properties and biological role of alpha-tocopherol binding proteins. Usp Sovrem Biol. 1993; 113(3):313-26.
[2] Rozen VB. [A superfamily of nuclear hormone receptors--adaptive transcription factors]. Biokhimiia. 1991;56(3):565-71.
[3] Donchenko GV. Ubiquinone biochemistry. Kiev: Naukova Dumka, 1988. 240 p.
[4] Minchenko AG, Dudareva NA. Mitochondrial genome. Novosibirsk: Nauka, 1990. 194 p.
[5] Petrova GV, Kapralov AA, Kuzmenko IV, Donchenko GV Investigation of tocopherol-binding proteins in membranes of the rat liver mitochondria. Ukr Biokhim Zh. 1990;62(2):29-35.
[6] Petrova GV, Kapralov AA, Izhokina IA, Donchenko GV. [Effect of alpha-tocopherol and ubiquinone on mitochondrial RNA polymerase activity. The role of tocopherol-binding proteins]. Biokhimiia. 1994;59(4):575-81.
[7] Boscoboinik D, Szewczyk A, Hensey C, Azzi A. Inhibition of cell proliferation by alpha-tocopherol. Role of protein kinase C. J Biol Chem. 1991;266(10):6188-94.
[8] Pal'mina NP, Mal'tseva EL, Kurnakova NV, Burlakova EB. [The effect of alpha-tocopherol over a wide range of concentrations (10(-2)-10(-17) M) on protein kinase C activity. Connection with proliferation and tumor growth]. Biokhimiia. 1994;59(2):193-200.
[9] Kleineke J, S?ling HD. Mitochondrial and extramitochondrial Ca2+ pools in the perfused rat liver. Mitochondria are not the origin of calcium mobilized by vasopressin. J Biol Chem. 1985;260(2):1040-5.
[10] Malviya AN, Block C. Nuclear protein kinase C and signal transduction. Receptor. 1993 Winter;3(4):257-75.
[11] Woodgett JR, Hunter T, Gould KL. Protein kinase C and its role in cell growth. Cell Membrane. Methods and reviews. Eds E. L. Elson, W. A. Frasier, R. Glaser-New York: Plenum Publ. Corp., 1987. Vol. 3: 1-88.
[12] Levitsky EL, Gubskiy JuI. Regulatory effect of calcium ions and cyclic nucleotides on DNA synthesis in mammalian cells. Biokhimiya zhivotnykh i cheloveka. 1990; 14: 33-44.
[13] Almazov A, Shlyahto EV. Ca antagonists and their use in hypertension. Intern Med Reviews. 1993; 1(4): 305-309.
[14] Avdonin PV, Tkachuk VA. Intracellular calcium receptors. M.: Nauka, 1994. 288 p.
[15] Parsons DF, Williams GR. Isolation and purification of the outer membrane and inner membrane of liver mitochondria. Methods Enzymol. 1967;443–8.
[16] Transcription and translation - a practical approach: Edited by B D Hames and S J Higgins. IRL Press, Oxford. 1984. 328 p.
[17] Donchenko GY, Kapralov AA, Petrova GY. Alfa-tocopherol prevents the inhibitory effects of A23187 and verapamil on RNA synthesis in isolated rat liver nuclei: Xllth Int. Congr. of Pharmacol. (July 1994). Can J Physiol Pharmacol. 1994; 72(1): 247.
[18] Maguire JJ, Wilson DS, Packer L. Mitochondrial electron transport-linked tocopheroxyl radical reduction. J Biol Chem. 1989;264(36):21462-5.
[19] Frei B, Kim MC, Ames BN. Ubiquinol-10 is an effective lipid-soluble antioxidant at physiological concentrations. Proc Natl Acad Sci U S A. 1990;87(12):4879-83.
[20] Aronica SM, Kraus WL, Katzenellenbogen BS. Estrogen action via the cAMP signaling pathway: stimulation of adenylate cyclase and cAMP-regulated gene transcription. Proc Natl Acad Sci U S A. 1994;91(18):8517-21. ttp://
[21] Moyer ML, Borror KC, Bona BJ, DeFranco DB, Nordeen SK. Modulation of cell signaling pathways can enhance or impair glucocorticoid-induced gene expression without altering the state of receptor phosphorylation. J Biol Chem. 1993;268(30):22933-40.
[22] Nemere I, Zhou LX, Norman AW. Nontranscriptional effects of steroid hormones. Receptor. 1993 Winter;3(4):277-91.
[23] Kashkarov KP, Vasil'eva EV, Ruuge EK. [Generation of superoxide radicals by the mitochondrial respiratory chain of isolated cardiomyocytes]. Biokhimiia. 1994;59(6):813-8.
[24] Shoji Y, Uedono Y, Ishikura H, Takeyama N, Tanaka T. DNA damage induced by tumour necrosis factor-alpha in L929 cells is mediated by mitochondrial oxygen radical formation. Immunology. 1995;84(4):543-8.
[25] Kristal BS, Chen J, Yu BP. Sensitivity of mitochondrial transcription to different free radical species. Free Radic Biol Med. 1994;16(3):323–9.