Biopolym. Cell. 1997; 13(3):202-208.
Structure and Function of Biopolymers
The primary structure of tRNASer from Thermus thermophilus
1Petrushenko Z. M., 1Kovalenko O. P., 1Malchenko N. N., 1Krikliviy I. A., 1Yaremchuk A. D., 1Tukalo M. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Sequences of two serine isoacceptor tRNASer from Thermus thermophilus, containing different anticodons have been studied. The homology of sequences is 60 %. The major differences have been found in anticodon and variable arms. Acceptor stems and T-arms have the same structures and D-arms differ by only one base pair.

References

[1] Asahara H, Himeno H, Tamura K, Nameki N, Hasegawa T, Shimizu M. Escherichia coli seryl-tRNA synthetase recognizes tRNA(Ser) by its characteristic tertiary structure. J Mol Biol. 1994;236(3):738-48.
[2] Asahara H, Himeno H, Tamura K, Hasegawa T, Watanabe K, Shimizu M. Recognition nucleotides of Escherichia coli tRNA(Leu) and its elements facilitating discrimination from tRNASer and tRNA(Tyr). J Mol Biol. 1993;231(2):219-29.
[3] Petrushenko ZM, Tukalo MA, Gudzera OI, Rozhko OT, Matsuka GKh. [Determination of interacting segments of tRNA(Leu) from cow mammary glands with homologous aminoacyl-tRNA-synthetase by a chemical modification method]. Bioorg Khim. 1990;16(12):1647-52.
[4] Dietrich A, Romby P, Mar?chal-Drouard L, Guillemaut P, Gieg? R. Solution conformation of several free tRNALeu species from bean, yeast and Escherichia coli and interaction of these tRNAs with bean cytoplasmic Leucyl-tRNA synthetase. A phosphate alkylation study with ethylnitrosourea. Nucleic Acids Res. 1990;18(9):2589-97.
[5] Tamura K, Asahara H, Himeno H, Hasegawa T, Shimizu M. Identity elements of Escherichia coli tRNA(Ala). J Mol Recognit. 1991;4(4):129-32.
[6] Davanloo P, Sprinzl M, Watanabe K, Albani M, Kersten H. Role of ribothymidine in the thermal stability of transfer RNA as monitored by proton magnetic resonance. Nucleic Acids Res. 1979;6(4):1571-81.
[7] Oshima T, Sakaki Y, Wakayama N, Watanabe K, Ohashi Z, Nishimura S. Biochemical Studies on an Extreme Thermophile Thermus thermophilus: Thermal Stabilities of Cell Constituents and a Bacteriophage. Experientia Supplementum. 1976;317–31.
[8] Watanabe K, Oshima T, Iijima K, Yamaizumi Z, Nishimura S. Purification and thermal stability of several amino acid-specific tRNAs from an extreme thermophile, Thermus thermophilus HB8. J Biochem. 1980;87(1):1-13.
[9] Watanabe K, Oshima T, Nishimura S. CD spectra of 5-methyl-2-thiouridine in tRNA-Met-f from an extreme thermophile. Nucleic Acids Res. 1976;3(7):1703-13.
[10] Horie N, Hara-Yokoyama M, Yokoyama S, Watanabe K, Kuchino Y, Nishimura S, Miyazawa T. Two tRNAIle1 species from an extreme thermophile, Thermus thermophilus HB8: effect of 2-thiolation of ribothymidine on the thermostability of tRNA. Biochemistry. 1985;24(21):5711-5.
[11] Watanabe K, Kuchino Y, Yamaizumi Z, Kato M, Oshima T, Nishimura S. Nucleotide sequence of formylmethionine tRNA from an extreme thermophile, Thermus thermophilus HB8. J Biochem. 1979;86(4):893-905.
[12] Bruce AG, Uhlenbeck OC. Reactions at the termini of tRNA with T4 RNA ligase. Nucleic Acids Res. 1978;5(10):3665-77.
[13] Venkstern T. The primary structure of transfer RNA. M.: Nauka, 1970. 258 p.
[14] Peattie DA. Direct chemical method for sequencing RNA. Proc Natl Acad Sci U S A. 1979;76(4):1760-4.
[15] Donis-Keller H, Maxam AM, Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977;4(8):2527-38.
[16] Vlasov VV, Grachev MA, Komarova NI, Kuz'min SV, Menzorova NI. Microscale ion-exchange chromatography and spectral analysis of oligonucleotides. Mol Biol. 1972;6(6):653-9.
[17] Gehrke CW, Kuo KC. Ribonucleoside analysis by reversed-phase high-performance liquid chromatography. J Chromatogr. 1989;471:3-36.
[18] Sprinzl M, Dank N, Nock S, Sch?n A. Compilation of tRNA sequences and sequences of tRNA genes. Nucleic Acids Res. 1991;19 Suppl:2127-71.
[19] Venegas A. T thermophllus HB8 tRNASer gene. EMBL data bank entry X07394. 1988.
[20] Himeno H, Hasegawa T, Ueda T, Watanabe K, Shimizu M. Conversion of aminoacylation specificity from tRNA(Tyr) to tRNA(Ser) in vitro. Nucleic Acids Res. 1990;18(23):6815-9.
[21] Biou V, Yaremchuk A, Tukalo M, Cusack S. The 2.9 A crystal structure of T. thermophilus seryl-tRNA synthetase complexed with tRNA(Ser). Science. 1994;263(5152):1404-10.
[22] Cusack S, Yaremchuk A, Tukalo M. The crystal structure of the ternary complex of T.thermophilus seryl-tRNA synthetase with tRNA(Ser) and a seryl-adenylate analogue reveals a conformational switch in the active site. EMBO J. 1996;15(11):2834-42.