Biopolym. Cell. 1987; 3(1):27-35.
http://dx.doi.org/10.7124/bc.0001D0
Genome and Its Regulation
Nuclear RNA at the early stage of liver regeneration
1Obolenskaya M. Yu., 1Prima V. I., 1Kulikovskaya I. A., 1Platonov O. M.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

During the first three hours of the rat liver regeneration two stages differing in synthesis, accumulation and qualitative characteristics of nuclear RNA are observed. At the first stage (0.5–1 h after operation) the synthesis of total and poly (A) + nuclear RNA, transcription of some unique DNA sequences and translocation of newly formed RNA from nucleus to cytoplasm are intensified. At the second stage (1–3 h after partial hepatectomy) the synthesis of total, poly (A) + nuclear RNA as well as transcription of some unique DNA sequences decrease while newly formed RNA is retained in nucleus. Nuclear RNA is characterized by a decrease in complexity of transcripts from unique part of genome with simultaneous increase in the number of some their copies and the growth of diversity of transcripts from middle repetitive sequences and relative content of transcripts from highly repeated DNA.
Full text: (PDF, in Russian)

References

[1] Terskikh VV. Rest periods in normal and malignant cell systems. Cell cycle. Moscow, Nauka, 1973; 165-89.
[2] Tsanev R. Cell cycle and liver function. Results and Problems in cell differentiation. Berlin et al. : Springer, 1975; 7:197-248.
[3] Zelenin AV, Kushch AA. Chromatin activation and various problems of genetic activity regulation in eukaryotic cells. Mol Biol (Mosk). 1985;19(1):285-94.
[4] Obolenskaya MYu, Prima VI, Gerasimova TB et al. Expression of the genome of early hepatocyte regeneration response. Comparative aspects of the study of regeneration and cell proliferation: Proc. of reports. VII all-union conf. Moscow, 1985. Part II.: 224-7.
[5] Iakovlev AIu. Hepatocyte dynamic reserve: the mechanism for the assurance of specialized functions in the regenerating liver. Tsitologiia. 1979;21(11):1243-52.
[6] Makino R, Hayashi K, Sugimura T. C-myc transcript is induced in rat liver at a very early stage of regeneration or by cycloheximide treatment. Nature. 1984 Aug 23-29;310(5979):697-8.
[7] Kaczmarek L, Hyland JK, Watt R, Rosenberg M, Baserga R. Microinjected c-myc as a competence factor. Science. 1985;228(4705):1313-5.
[8] Hirschhorn RR, Aller P, Yuan ZA, Gibson CW, Baserga R. Cell-cycle-specific cDNAs from mammalian cells temperature sensitive for growth. Proc Natl Acad Sci U S A. 1984;81(19):6004-8.
[9] Grady LJ, Campbell WP, North AB. Nonrepetitive DNA transcription in normal and regenerating rat liver. Nucleic Acids Res. 1979;7(1):259-69.
[10] Grady LJ, Campbell WP, North AB. Sequence diversity of nuclear and polysomal polyadenylated and non-polyadenylated RNA in normal and regenerating rat liver. Eur J Biochem. 1981;115(2):241-5.
[11] Krieg L, Alonso A, Volm M. Kinetic complexity of nuclear poly(A)-containing RNA in normal and regenerating rat liver. Eur J Biochem. 1979;96(1):77-85.
[12] Platonov OM, Lisitsa EG. Some features of nuclear genome transcription at early stages of liver regeneration. Nuclear DNA-like RNAs, complementary to uniquie and repeating sequences of DNA. Biokhimiia. 1980;45(5):896-900.
[13] Prima VI, Lisitsa EG, Platonov OM. Features of nuclear genome transcription at early steps of liver regeneration: expression of DNA fractions with different repeating sequence frequencies. Biokhimiia. 1982;47(1):145-52.
[14] Higgins GM, Anderson RM. Experimental pathology of the liver. Arch Pathol. 1931; 12(2):186-202.
[15] Obolenskaya MYu, Prima VI, Platonov OM. Hybridization of nucleic acids into eukaryotic presence of high concentrations of formamide. Stud. biophys. 1983; 98(2):103-10.
[16] Maniatis T., Fritsch E. F., Sambrook J. Molecular cloning: a laboratory manual New York: Cold Spring Harbor Lab. publ., 1982 545 p.
[17] Prima VI, Platonov OM, Gazarian KG. Reassociation of rat DNA fractions. Biokhimiia. 1980;45(3):498-506.
[18] Chikaraishi DM, Deeb SS, Sueoka N. Sequence complexity of nuclear RNAs in adult rat tissues. Cell. 1978;13(1):111-20.
[19] Paetkau V, Langman L. A quantitative, batch hydroxyapatite method for analyzing native and denatured DNA at room temperature. Anal Biochem. 1975;65(1-2):525-32.
[20] Britten RJ, Graham DE, Neufeld BR. Analysis of repeating DNA sequences by reassociation. Methods Enzymol. 1974;29:363-418.
[21] Chernovskaia TV, Liubimova EV, Lerman MI. Metabolism of mRNA in regenerating mouse liver: acceleration of the processing and decay of mRNA. Mol Biol (Mosk). 1976;10(6):1361-8.
[22] Holland CA, Mayrand S, Pederson T. Sequence complexity of nuclear and messenger RNA in HeLa cells. J Mol Biol. 1980;138(4):755-78.
[23] Greene RF, Fausto N. Analysis of gene expression in regenerating rat liver by hybridization of nuclear and cytoplasmic RNA with DNA. Cancer Res. 1977;37(1):118-27.
[24] Platonov OM, Gerasimova TB, Andrianov VM, Smal'ko PIa, Korniushina TV. Changes in the cytoplasmic ultrastructure and polyribosome content of rat liver cells in the 1st hours after a partial hepatectomy. Tsitol Genet. 1981;15(5):25-8.
[25] Gerasimova TB, Obolenskaia MIu, Platonov OM. Autoradiographic study of RNA synthesis and secretion into the cytoplasm during the early stages of liver regeneration. Tsitol Genet. 1980;14(3):77-82.
[26] Davidson EH, Britten RJ. Regulation of gene expression: possible role of repetitive sequences. Science. 1979;204(4397):1052-9.
[27] Gasaryan KG, Tarantul WZ, Baranov YN. Transcription of repetitive and unique DNA nucleotide sequences in pigeon erythroid cells with different degrees of specialization. Differentiation. 1976;6(1):41-6.
[28] Khandekar P, Saidapet C, Krauskopf M, Zarraga AM, Lin WL, Mendola C, Siddiqui MA. Co-ordinate control of gene expression. Muscle-specific 7 S RNA contains sequences homologous to 3'-untranslated regions of myosin genes and repetitive DNA. J Mol Biol. 1984;180(3):417-35.