Study of Poly(U) and poly(dT)-dependent Phe-tRNAPhe binding to 30S subunits of Escherichia coli ribosomes

Soldatkin, K. A.; Potapov, A. P.; Soldatkin, A. P.; El'skaya, A. V.

doi:10.7124/bc.000229

Biopolym. Cell. 1988; 4(4):193-196.

http://dx.doi.org/10.7124/bc.000229

Structure and Function of Biopolymers

Study of Poly(U) and poly(dT)-dependent Phe-tRNA^Phe binding to 30S subunits of Escherichia coli ribosomes

1Soldatkin K. A., 1Potapov A. P., 1Soldatkin A. P., 1El'skaya A. V.

Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
Kiev, USSR

Abstract

To study the role of the codon sugar-phosphate backbones in the mRNA decoding process we have compared the messenger activity of authentic ribo- and deoxyribopolynucle-otides, poly(U) and poly(dT), in the factor-free binding of Phe-tRNA^Phe to the SOS subunits of E. coli ribosomes. The template efficiency of poly(U) is much higher than that of poly(dT). Template 2'-hydroxyl groups seem to be important for the binding process at the A site of SOS subunit. The results obtained indicate the co-operative nature of the occupation of the P and A sites of SOS subunits. Neomycin exerted an insignificant effect upon the Phe-tRNA^Phe binding. High sensitivity of translation efficiency to neomycin may be due to the translocation stage.

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