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Search for the modification-restriction systems in Agrobacteria DNA
- Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
- Institute of Biochemistry and Physiology of Microorganisms, Academy of Sciences of the USSR
Pushchino, Moscow Region, USSR
The DNA modification-restriction systems have been studied for their presence in 19 Agrobacieria strains, including the virulent ones with 77-plasmids of various classes, the avirulent octopine and nopaline strain derivatives obtained by curing the Agrobacteria virulent strains from 77-plasmids as well as natural isolates of avirulent Agrobacteria. It is shown that the presence of the DNA modification-restriction system with EcoRIl specificity is a characteristic feature of the Agrobacteria octopine strains and of their cured avirulent derivatives. The Agrobacteria nopaline strains, their cured avirulent derivatives, and most of the Agrobacteria natural avirulent strains contain the DNA modification-restriction systems different from EcoRIl. The chromosomal nature of these characters is shown.
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