Biopolymers and Cell. 2012; 28(4):322-325
THE LEVEL OF BLOOD PLASMA MITOCHONDRIAL DNA UPON ACUTE MYOCARDIUM DAMAGE IN EXPERIMENT
1, 3Sudakov N. P., 3Popkova T. P., 1Novikova M. A.,2 Katyshev A. I., 1Nikiforov S. B., 1Pushkarev B. G., 1Goldberg O. A., 4, 6Klimenkov I. B., 1Lepekhova S. A., 3Ezhikeeva S. D., 3Ten M. N., 5Osipov V. G., 2, 6Konstantinov Yu. M.
1Scientific Center for Reconstructive and Restorative Surgery, Siberian Branch, Russian Academy of Medical Sciences
1, Bortsov Revolyutsii Str., Irkutsk, Russian Federation, 664003
2Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences
132, Lermontova Str., Irkutsk, Russian Federation, 664033
3Regional Clinical Hospital
100, m-n Yubileinyi, Irkutsk, Russian Federation, 66409
4Limnological Institute, Siberian Branch of the Russian Academy of Sciences
3, Ulan-Batorskaya Str., Irkutsk, Russian Federation, 66503
5Territorial Center of Catastrophe Medicine
100 k3, m-n Yubileinyi, Irkutsk, Russian Federation, 664049
6Irkutsk State University
1, Karl Marks Str., Irkutsk, Russian Federation, 664003
The aim of the present investigation is to study the level of plasma mtDNA as a potential marker of cardiomyocyte damage in 2 and 4 h after subcutaneous injection of adrenaline and during the formed morphological alterations of the myocardium (3 days). Methods. Real-time PCR. Male Wistar rats were used as the experimental animals. Results. It was shown that during the increase in the activity of cytolysis biomarkers, at the first hours after adrenaline injection, no reliable increase is observed in the level of free circulating blood mtDNA. A tendency of 2.5-fold increase in this parameter was established at the third day after adrenaline injection during the development of acute inflammatory process in the myocardium. On the whole, further researches are needed on the dynamics of mtDNA level upon acute damage of the myocardium in experimental and clinical investigations for unbiased estimation of the prospects of using the parameter in laboratory diagnostics |
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