Biopolymers and Cell. 2011; 27(4): 279-284

 

 

EXPRESSION OF THE SUBUNITS OF MULTIPROTEIN TRANSLATION ELONGATION COMPLEX EEF1H IN HUMAN RENAL CARCINOMAS

 

Veremieva M. V., 1Zgonnik Yu. M., Negrutskii B. S., El’skaya A. V.

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680
1State Institution "Institute of Urology of NAMS of Ukraine"
9-a, Yu. Kotsubyns'koho Str., Kyiv, Ukraine, 04053

 

    Aim. To investigate the expression of all eEF1 complex subunits at the mRNA and protein levels in human renal cancer. Methods. The protein content and mRNA level of the eEF1 complex components were analyzed in 18 clinical human renal carcinoma specimens and correspondingly paired normal tissues by Western blot and Northern blot techniques. Results. The changes in the level of protein components of the eEF1 complex or mRNAs coding for these proteins in human renal carcinoma specimens were found to be not essential. Conclusions. This study suggests that eEF1 subunits have no additional cancer-related functions in renal tissue. The data indicate that the occurrence of cancer-induced changes in the eEF1 subunits expression may depend on the tumor localization

 

Keywords: protein synthesis, translation elongation factor eEF1H, human renal cell carcimoma

 

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