Biopolymers and Cell. 2011; 27(3): 231-234

IFN-λ- 3 (IL28B) GENOTYPING BY RESTRICTION FRAGMENT LENGTH POLYMORPHISM METHOD: DETECTION POLYMORPHISM OF RS12979860

V. M. Pampukha, S. A. Kravchenko, L. V. Moroz ¹, L. A. Livshits

Institute of Molecular Biology and Genetics, NAS of Ukraine
150, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680

¹National Pirogov Memorial Medical University NAMS of Ukraine
56, Pirogova St., Vinnytsya, Ukraine, 21018

    Aim. The goal of our study was to develop an accurate detection of the SNP rs12979860 by RFLP-based method and to evaluate the polymorphic genotype distribution for this SNP among individuals with unknown HCV status from Ukraine. Methods.The SNP rs12979860 was tested by PCR RFLP-based method in 99 individuals from Ukraine. Results. The method of accurate detection of the SNP rs12979860 was developed. The genotypes distributions were: CC – 56 %, CT – 34 %, TT – 10 %. Conclusions. Due to the high incidence of CC genotype, found in our study, the SNP rs12979860 analysis may be useful for Ukrainian patients to predict responses to the treatment considering the HCV genotype and viral load.

Keywords: IFN- λ -3 (IL28B) gene, SNP rs12979860, PCR-RFLP method, hepatitis C.

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