Biopolymers and Cell. 2011; 27 (1): 36-39
Hsp60 and p70S6K form a complex in human cardiomyocytes
I. V. Kroupskaya, L. N. Kapustian, I. O. Tykhonkova, L. L. Sidorik
Institute of Molecular Biology and Genetics NAS of Ukraine
150, Akademika Zabolotnogo St., Kyiv, Ukraine, 03680
Molecular chaperon Hsp60 and protein kinase p70S6K play an important functional role in the regulation of cardiomyocytes vital function or apoptosis. Aim. To study a possibility of in vivo complex formation between Hsp60 and p70S6K in cardiomyocytes. Methods. Co-immunoprecipitation, Western-blot analysis. Results. We have identified in vivo interaction between molecular chaperone Hsp60 and two isoforms of proteinkinase p70S6K in human myocardium, normal and affected by cardiomyopathy. Conclusions. The results obtained suggest a possible participation of molecular chaperon Hsp60 in regulation of p70S6K activity in stressinduced apoptotic signaling pathway in cardiomyocytes.
Keywords: Hsp60, p70S6K, cardiomyocyte, DCM, apoptosis, co-immunoprecipitation