Biopolymers and Cell. 2010; 26 (5): 373-377

 

 

Identification of autoantibodies to tyrosil-tRNA synthetase in heart disfunctions

 

Iu. Iu. Kondratiuk1, 2, L. L.Sidorik1, V. I. Bobyk1, D. V. Ryabenko3, A. I. Kornelyuk1

 

1Institute of Molecular Biology and Genetics, NAS of Ukraine; 150, Zabolotnoho Str., Kyiv, Ukraine, 03680

2Taras Shevchenko National University of Kyiv; 64, Volodymyrska Str., Kyiv, Ukraine, 01033

3National Scientific Centre «M. Strazhesko Institute of Cardiology» AMS of Ukraine; 5, Narodnoho Opolchennya Str., Kyiv, Ukraine, 03151

 

Aim. To investigate the levels of specific autoantibodies against tyrosyl-tRNA synthetase and its individual modules in the blood serum of people with heart failure caused by dilated cardiomyopathy, myocarditis and ischemic heart disease compared with healthy donors. Methods. Recombinant proteins were obtained using bacterial strains transformed with appropriate plasmid vectors and were purified by chromatography on Ni-NTA-agarose. The levels of specific autoantibodies were investigated by ELISA. Results. The increased levels of autoantibodies specific to tyrosyl-tRNA synthetase, its N-terminal catalytic module and non-catalytic C-module, were found in the blood serum of patients, compared with healthy donors. Conclusions. The results obtained demonstrate the possible role of tyrosyl-tRNA synthetase in adaptive changes of the myocardium in response to stress factors.

 

Keywords: autoantibodies, tyrosyl-tRNA synthetase, heart failure.

 

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