Biopolymers and cell. 2010; 26 (4): 322 - 326
Comparative analysis of human and mammalias genes of protein ortologs of cytochrome P450 2E1
D. R. Duplij, O. V. Maksymchuk, V. P. Duplij1, N. O. Chaschin
Institute of molecular biology and genetics NAS of Ukraine 150, Zabolotnogo Str, Kyiv Ukraine, 03680
1 Institute of cell biology and genetic engineering
NAS of Ukraine, 148, Zabolotnogo Str, Kyiv Ukraine, 03680
Aim. To carry out a comparison analysis of nucleotide sequences for cytochrome P450 2E1 protein ortologs genes and reestablish a
connection of gene evolution with nucleotide content. Methods. In silico: BLAST, ClustalW, MEGA4, PHP. Results. A general phylogeny of CYP2E1 genes is described. The most affinity is found for human translated sequences with Pan troglodytes. The transition C>T is the most often, it occurs in introns in 2.6 more times than in exons. The correlation of keto-amino skew of CYP2E1 genes and evolution age of species. Conclusions. The analysis carried out in our paper allows us to assume that a common ancestor of CYP2E1 protein isoform lived before divergence between rodent and Primates orders, i.e. 70 millions years ago. The single nucleotide substitution are accumulated in introns during evolution.
Keywords: cytochrome P450 2E1, CYP2E1, transition, phylogeny, nucleotide composition skew.