Biopolymers and cell. 2009; 25 (6): 484 - 490
Newly synthesized carbocyanine fluorescent probes, their characteristics and behavior in proliferating cultures
E. I. Goncharuk, I. A. Borovoy1, E. V. Pavlovich, Yu. V. Malyukin1, V. I. Grischenko
Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine 23, Pereyaslavskaya Str., Kharkiv, Ukraine, 61015
1State Scientific Institution «Institute for Single Crystals» NAS of Ukraine 60, Lenin Ave.,Kharkiv, Ukraine, 61001
Aim. To study possibile application of C2, C9, C18 and JC-1 carbocyanine fluorescent dyes for cell culture characterization. Methods. Morphological methods, fluorescence-activated cell sorting (FACS) analysis, luminescent microscopy were used. Results. The studied carbocyanine probes were shown to be preserved in dividing cells for at least 4 duplications. It was found that carbocyanine probe JC-1 did not transit from cell to cell under combined culturing of labeled and non-labeled cells. Conclusions. The paper covers the use of carbocyanine fluorescent probes for long-term culturing of cell lines. Probes C9 and JC-1 were optimal for the proliferative culture observation, allowing to trace mitochondrial functional state.
Key words: fluorescence, probes, human fibroblasts, cell culture.