Biopolymers and cell. 2009; 25 (4): 315 - 318
Study on toxin-neutralization properties of recombinant single-chain variable antibody’s fragments against diphtheria toxin B subunit
O. S. Oliinyk, A. J. Labyntsev, N. V. Korotkevich, D. V. Kolibo, S. V. Komisarenko
Palladin Institute of Biochemistry of the NAS of Ukraine
9 Leontovicha Str., Kyiv, Ukraine, 01601
Aim. To select scFv which have an ability to neutralize diphtheria toxin. Methods. Flow-cytometry was
used to study the ability of specific scFv to block the binding of a labeled receptor binding fragment of toxin
with a surface of sensitive to toxin cells. Results. Four scFv with toxin-neutralization properties
were selected. Conclusions. The obtained scFv could be considered as promising candidates for antitoxic antidiphtheria drugs of new generation.
Keywords: antitoxins, scFv, diphtheria toxin.