Biopolymers and cell. 2009; 25 (3): 194 - 203

Application of L-lactate-cytochrome c-oxidoreductase for development of amperometric biosensor for L-lactate determination

T. B. Goriushkina, A. P. Orlova, O. V. Smutok, M. V. Gonchar, A. P. Soldatkin, S. V. Dzyadevych

National Taras Shevchenko University of Kyiv 64, Volodymyrska Str, Kyiv, Ukraine 01033

Institute of Cell Biology NAS of Ukraine 14/16, Drahomanov St., Lviv, Ukraine, 79005

Zaklad Biotechnologii, Zamiejscowy Wydzial Biotechnologii, Uniwersytet Rzeszowski Kolbuszowa, Polska

Aim. Development of amperometric biosensor based on L-lactate-cytochrome c-oxidoreductase (flavocytochrome b2, FC b2) for lactate determination. Methods. All experiments were performed using the amperometric method of detection. The methods of electrochemical polymerization and immobilization in glutaraldehyde vapors were used for FC b2 immobilization on the surface of electrodes. Results. The FC b2 preparation, which demonstrated the best operational characteristics after immobilization in poly (3,4-ethylen dioxythiophene), was selected. The selectivity, operational and storage stability, and pH-optimum for operation of the created biosensor were determined. The analysis of L-lactate in the model solutions and wine samples was carried outusing the developed biosensor. Conclusion. The FC b2-based biosensor due to its high stability can be effectively used for lactate determination in blood and other liquids containing no ethanol. After the selectivity optimization, the devise can be also applied for wine analysis.

Keywords: amperometric biosensor, flavocytochrome b2, L-lactate.