Biopolymers and cell. 2009; 25 (2): 120 - 125

Agrobacterium-mediated transformation of Cichorium intybus L. with interferon-a2b gene

N. A. Matvieieva, A. M. Shachovsky, I. M. Gerasymenko, O. Yu. Kvasko, N. V. Kuchuk

Institute of cell biology and genetic engineering NAS of Ukraine 148, Zabolotnogo Str,  Kyiv  Ukraine, 03680

An efficient method for the plant regeneration and Agrobacterium mediated transformation with interferon-a2b gene has been developed for chicory C. intybus L. cv. Pala rossa. The regeneration with efficiency about 100 % was induced on the MS medium supplemented with 0.5–2.5 mg/l kinetin and 0.5 mg/l NAA. The transformed plantlets were recovered at a frequency 26,9 % on basal medium with 25 mg/l kanamycin. According to PCR-analysis the nptII and ifn-a2b genes were integrated into the genome of transformed plants.

Keywords: transformation, Agrobacterium, Cichorium intybus, interferon.