Biopolymers and cell. 2009; 25 (2): 95 - 100

Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer

V. S. Gryshkova, D. S. Lituyev, V. V. Filonenko, R. G. Kiyamova

Institute of Molecular Biology abd Genetics, National Academy of Sciences of Ukraine 150 Academician Zabolotnogo str., Kyiv, Ukraine, 03680

The aim of present study was to develop a model for a functional analysis of a recently identified marker of the ovarian cancer – sodium-dependent phosphate transporter NaPi2b. For this purpose, we have created HEK293 stable cell lines expressing wild type or mutant forms of NaPi2b (T330V substitution in a large extracellular loop and a 6 amino acid residues deletion in the C-terminal cytoplasmic tail), revealed in the ovarian cancer cell lines. The expression of wild type and mutant forms NaPi2b in the stable cell lines created was confirmed by Western-blot analysis with monoclonal antibodies against NaPi2b. The cellular models described here will be useful for studying the function of sodium-dependent phosphate transporter NaPi2b in health and disease.

Keywords: sodium-dependent phosphate transporter NaPi2b, mutation, anti-NaPi2b MAb.